PIK3CA Knockout Hep-G2 Cell Line
Cat.No.:
EDJ-KQ40
Species:
Human
Cell Name:
Hep-G2
Gene:
PIK3CA
Gene ID:
5290
Size:
1×10⁶cells
PIK3CA Knockout Cell Line (HEPG2) is an exclusive upgraded CRISPR/Cas9 system-mediated gene knockout cell, with the advantages of Optimized Strategy Design, Efficient Cell Transfection, High-Performotion Cas9 Protein and Hassle-Free Cell Selection.
| Cat.No. | EDJ-KQ40 |
|---|---|
| Product Name | PIK3CA Knockout Cell Line (HEPG2) |
| Cell line | Hep-G2 |
| Cellosaurus ID | CVCL_0027 |
| Cell Line Synonyms | HEP-G2, Hep G2, HEP G2, HepG2, HEPG2 |
| Gene | PIK3CA |
| NCBI Gene ID | |
| Gene Synonyms | CCM4|CLAPO|CLOVE|CWS5|HMH|MCAP|MCM|MCMTC|PI3K|PI3K-alpha|p110-alpha |
| Summary |
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. [provided by RefSeq, Apr 2016]
|
| Associated Diseases | Hepatocellular Carcinoma |
| Morphology | Adherent |
| Passage Ratio | 1:2-1:3 |
| Complete Culture Medium | DMEM+10%FBS |
| Freezing Medium | 55% Basal medium +40%FBS+5%DMSO |
| QC | Indels validated by Sanger sequencing; sterility confirmed via microbial testing. |
* For research use only. Not intended for use in humans or animals, including clinical, therapeutic, or diagnostic purposes.
| Loci | STR Info (Sample Cell) Sample Cell Line: Hep-G2 | STR Info (Cell bank) Cell Line: Hep-G2 | ||
| Allele1 | Allele2 | Allele1 | Allele2 | |
| Amelogenin | X | Y | X | Y |
| CSF1P0 | 10 | 11 | 10 | 11 |
| D2S1338 | 19 | 20 | 19 | 20 |
| D3S1358 | 15 | 16 | 15 | 16 |
| D5S818 | 11 | 12 | 11 | 12 |
| D7S820 | 10 | 10 | ||
| D8S1179 | 15 | 16 | 15 | 16 |
| D13S317 | 9 | 13 | 9 | 13 |
| D16S539 | 12 | 12 | 13 | |
| D18S51 | 13 | 14 | 13 | 14 |
| D19S433 | 15.2 | 15.2 | ||
| D21S11 | 29 | 31 | 29 | 31 |
| FGA | 22 | 25 | 22 | 25 |
| Penta D | 9 | 13 | 9 | 13 |
| Penta E | 15 | 20 | 15 | 20 |
| TH01 | 9 | 9 | ||
| TPOX | 8 | 9 | 8 | 9 |
| vWA | 17 | 17 | ||
| D6S1043 | 13 | |||
| D12S391 | 21 | 25 | 21 | 25 |
| D2S441 | 11.3 | 14 | 11.3 | 14 |
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.
Conclusion: The STR identification of this cell is correct.
* Research Use Disclaimer: Content is generated from publicly available research data, bioinformatic resources, and computational analyses for research reference only.
Required Accessories
Cell Culture Optimization Series
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