Epitope Tag Knock-in
Precise knock-in of epitope tags at endogenous loci using CRISPR/Cas9 enables stable and reproducible protein detection and purification.Compared to reliance on endogenous antibodies or overexpression systems, Epitope Tag Knock-in provides a consistent antibody recognition site and physiological expression levels, significantly reducing experimental variability.Based on the FLASH-KI™ technology platform, we achieve efficient co-delivery of Cas9 RNP and Donor templates, improving knock-in efficiency and success rates while maintaining ≥90% cell viability.
Service Details
| Cell Types | Wide range including tumor cell lines, normal somatic cell lines, stem cells, etc. |
|---|---|
| Services | Single-gene Epitope Tag knock-in (FLAG, HA, Myc, V5, His, etc.), custom tag and site design |
| Deliverables | 1 monoclonal cell line + parental cell line (2 vials/cell line, 1×10^6 cells/vial) |
| Turnaround / Price |
 Consult online for details |
Service Advantages
Standardized Protein Detection
Uniform antibody recognition (FLAG / HA / Myc)
Avoids batch-to-batch antibody variability
Avoids batch-to-batch antibody variability
More Reliable Endogenous Expression
Preserves native regulation
Avoids overexpression artifacts
Avoids overexpression artifacts
Enhanced Experimental Reproducibility
More consistent WB / IP / ChIP results
Reduced experimental variation
Reduced experimental variation
FLASH-KI™ Efficient Delivery
Cas9 RNP + Donor co-delivery
No electroporation/lipofection required
Suitable for hard-to-transfect cells
No electroporation/lipofection required
Suitable for hard-to-transfect cells
Tag Types and Applications
| Tags | FLAG, HA, Myc, V5, His |
|---|---|
| Insertion position | N-terminal / C-terminal |
| Applications | Immunoprecipitation (Co-IP), Western Blot, Chromatin Immunoprecipitation (ChIP), Protein purification, Protein interaction studies |
| Features | No specific antibody required; high detection consistency |
Workflow
Advantage and Characteristic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
FAQ
Is it compatible with ChIP assays?
Yes; HA/FLAG tags are widely used in ChIP.
Is it suitable for low-abundance proteins?
Yes; tags can increase detection sensitivity.
Why not use endogenous antibodies?
Endogenous antibodies suffer from poor specificity and batch-to-batch variability; Epitope Tag provides a uniform detection standard.
Does the tag affect protein function?
- C-terminal design is preferred
- Linkers are used to minimize impact
- Functional validation options are available
