Functional Tag Knock-in
Precise knock-in of functional tags at endogenous loci using CRISPR/Cas9 enables controlled degradation, labeling, regulation, and interaction analysis of proteins.Compared to traditional overexpression or exogenous systems, Functional Tag Knock-in allows controllable functional modulation at native expression levels, making it suitable for drug discovery and advanced mechanistic studies.Based on the FLASH-KI™ technology platform, we achieve efficient co-delivery of Cas9 RNP and Donor templates, improving knock-in efficiency and stability while maintaining ≥90% cell viability.
Service Details
| Cell Types | Wide range including tumor cell lines, normal somatic cell lines, stem cells, etc. |
|---|---|
| Services | Functional Tag knock-in (Halo-Tag, SNAP-Tag, Degron, Biotin, etc.), custom functional models |
| Deliverables | 1 monoclonal cell line + parental cell line (2 vials/cell line, 1×10^6 cells/vial) |
| Turnaround / Price |
 Consult online for details |
Service Advantages
Controllable Protein Function
Degron system enables inducible degradation
Precise control of protein levels
Precise control of protein levels
Multi-functional expansion
HaloTag/SNAP for labeling
BioID for proximity labeling
BioID for proximity labeling
Endogenous Expression System
Retains natural regulation
Avoids overexpression interference
Avoids overexpression interference
FLASH-KI™ Efficient Delivery
Cas9 RNP + Donor co-delivery
No electroporation or viral vectors required
Suitable for hard-to-transfect cells
No electroporation or viral vectors required
Suitable for hard-to-transfect cells
Tag Types and Applications
| Tags | Halo-Tag, SNAP-Tag, Degron, Biotin |
|---|---|
| Insertion position | N-terminal / C-terminal (based on functional requirements) |
| Applications | Conditional protein knockdown (degron), optogenetic labeling, proximity labeling (BioID/TurboID), ubiquitination studies, live-cell functional labeling, drug target validation |
| Features | Enables functional modulation and expands research dimensions |
Workflow
Advantage and Characteristic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
FAQ
Do you support multi-tag combinations?
Yes, e.g., HaloTag + Degron.
Is it suitable for drug discovery studies?
Yes, commonly used for target validation.
Will the tag affect protein function?
Impact is minimized by optimizing insertion position and linker design.
How does the Degron system control proteins?
Controllable degradation induced by small molecules
