Knock-in Tag
In gene function research and cell therapy development, endogenous tag knock-in is a critical approach for analyzing protein localization, expression regulation, and functional dynamics. Compared to random integration or plasmid transfection, Knock-in Tag enables stable expression and quantitative analysis under native gene regulation.
Based on our proprietary FLASH-KI™ technology platform, we achieve efficient co-delivery of CRISPR/Cas9 RNP and Donor templates, supporting precise knock-in of multiple tag types and enabling the generation of high-consistency cell models.
Based on our proprietary FLASH-KI™ technology platform, we achieve efficient co-delivery of CRISPR/Cas9 RNP and Donor templates, supporting precise knock-in of multiple tag types and enabling the generation of high-consistency cell models.
Cell Types
Wide range including tumor cell lines, normal somatic cell lines, stem cells, etc.
Services
Fluorescent Tag, Epitope Tag, Reporter Tag, Functional Tag knock-in
Deliverables
1 monoclonal cell line + parental cell line (2 vials/cell line, 1×10^6 cells/vial)
Turnaround / Price
Consult online for details
Consult online for details
Advantage and Characteristic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
FAQ
What are the advantages of FLASH-KI™ over ssODN or AAV delivery?
FLASH enables efficient co-delivery of RNP and long-fragment donors without electroporation or AAV packaging; lower cell damage and significantly higher HDR efficiency than conventional methods.
Which cell types are suitable?
Successfully validated in CHO-K1, HEK293, HeLa, A549, iPSC, organoids, etc. Particularly outstanding performance in hard-to-transfect cells.
Will tag knock-in affect endogenous protein function?
We complement missing amino acids at the cut site and prioritize insertion in non-critical domains (e.g., via flexible linkers) or 3'UTR; a "self-cleaving peptide" (P2A/T2A) strategy is also available to co-express tag and protein without fusion.
Do you support large fragment tag knock-in?
Yes. FLASH can deliver long circular donors; we have successfully completed knock-in of a 3.5 kb complete Luciferase expression cassette.
