Product Description
Mycoplasma is a group of the smallest and simplest prokaryotic organisms. Mycoplasma infection alters the DNA, RNA, and protein expression of cells, leading to slow cell growth rates, morphological changes, and other pathological alterations, which significantly affect experimental results. Detection and prevention of Mycoplasma contamination is an essential step in cell experiments. This kit works by amplifying the conserved region of the Mycoplasma genome using specific primers. After PCR amplification, the presence of Mycoplasma contamination can be confirmed through electrophoresis. This method is characterized by high sensitivity, strong specificity, and rapid detection.
Product Components
| Reagent | Storage Conditions | 50T(EDMD-01) | 100T(EDMD-02) |
| MycoD Primer MIX | -20℃ | 100μL | 200 μL |
| MycoD Control template | -20℃ | 100 μL | 200 μL |
| MycoD Master Mix | -20℃ | 750 μL | 1.5 mL |
| MycoD Proteinase K Solution | -20℃ | 50 μL | 100 μL |
Workflow Diagram
Figure 1. Schematic Diagram of the Mycoplasma Detection Workflow
Work Areas
Figure 2. Suggested Division of Experimental Areas
Note: The use of a biosafety cabinet depends on the laboratory environment
Storage
Shipped with ice packs; store at -20°C with a shelf life of 12 months.
