Reporter Tag Knock-in
Precise knock-in of reporter genes at endogenous loci using CRISPR/Cas9 enables real-time quantitative monitoring of gene expression and signaling pathway analysis.Compared to transient transfection or random integration systems, Reporter Tag Knock-in offers stable expression (single-copy integration), low background signal, and high data reproducibility.Based on the FLASH-KI™ technology platform, we achieve efficient co-delivery of Cas9 RNP and Donor templates, improving knock-in efficiency and stability while maintaining ≥90% cell viability.
Service Details
| Cell Types | Wide range including tumor cell lines, normal somatic cell lines, stem cells, etc. |
|---|---|
| Services | Reporter Tag knock-in (Luciferase Firefly/Renilla, HiBiT, etc.), high-throughput screening model construction |
| Deliverables | 1 monoclonal cell line + parental cell line (2 vials/cell line, 1×10^6 cells/vial) |
| Turnaround / Price |
 Consult online for details |
Why Choose Our Knock-in Tag Service?
Core Performance Metrics
| Metric | Value |
|---|---|
| Knock-in efficiency | 45%–88% |
| Cell viability | ≥90% |
| Project success rate | 83% |
| Completed Projects | 100+ |
Service Advantages
Stable expression system
Single-copy integration
Long-term stable expression
Long-term stable expression
Quantitative analysis capability
Real-time dynamic monitoring
Suitable for automated platforms
Suitable for automated platforms
High-sensitivity detection
Luciferase / HiBiT systems
Low background, high signal-to-noise ratio
Low background, high signal-to-noise ratio
FLASH-KI™ Efficient Delivery
No electroporation/viral vectors required
RNP + Donor co-delivery
Suitable for hard-to-transfect cells
RNP + Donor co-delivery
Suitable for hard-to-transfect cells
Tag Types and Applications
| Tags | Luciferase (Firefly/Renilla), HiBiT |
|---|---|
| Insertion position | 3'UTR or ORF |
| Applications | Signaling pathway analysis, high-throughput screening, in vivo imaging, promoter activity assays, gene expression tracking |
| Features | High sensitivity, quantifiable, suitable for automated platforms |
Workflow
Case Study
Site-specific Luciferase knock-in at the AAVS1 safe harbor locus in CHO-K1 cells
Goal: Luciferase reporter knock-in at the CHO-K1 safe harbor locus for long-term in vivo bioluminescence tracing.
Approach: FLASH-KI™ delivery of Cas9 RNP + Donor vector containing Luciferase.
Result: Single-copy site-specific integration clone obtained; high and stable luminescence in vitro; used by customer for biodistribution studies of cell therapy products.
Approach: FLASH-KI™ delivery of Cas9 RNP + Donor vector containing Luciferase.
Result: Single-copy site-specific integration clone obtained; high and stable luminescence in vitro; used by customer for biodistribution studies of cell therapy products.
| Sample | Replicate 1 | Replicate 2 | Replicate 3 | Mean | Expression Fold |
|---|---|---|---|---|---|
| WT | 750 | 978 | 1402 | 1043 | 226 |
Advantage and Characteristic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
FAQ
Can dual-reporter systems be generated?
Yes, Dual-luciferase designs are supported.
What are the advantages of the Reporter system over transient transfection?
Stable expression
high data consistency
suitable for long-term experiments
high data consistency
suitable for long-term experiments
Is it suitable for in vivo experiments?
Yes, supports bioluminescence imaging.
Does it support high-throughput screening?
Yes, suitable for automated screening platforms.
