TEAD1 Knockout HCT 116 Cell Line

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TEAD1 Knockout HCT 116 Cell Line
Cat.No.:

EDJ-KQ20921

Species:

Human

Cell Name:

HCT 116

Gene:

TEAD1

Gene ID:

7003

Size:

1×10⁶cells

TEAD1 Knockout Cell Line (HCT116) is an exclusive upgraded CRISPR/Cas9 system-mediated gene knockout cell, with the advantages of Optimized Strategy Design, Efficient Cell Transfection, High-Performance Cas9 Protein and Hassle-Free Cell Selection.
Cat.No. EDJ-KQ20921
Product Name TEAD1 Knockout HCT 116 Cell Line
Cell Line HCT 116
Cellosaurus ID CVCL_0291
Cell Line Synonyms HCT-116, HCT.116, HCT_116, HCT116, HCT116wt, HCT-116/P, HCT-116/parental, CoCL2
Gene TEAD1
NCBI Gene ID
Gene Synonyms AA|NTEF-1|REF1|TCF-13|TCF13|TEAD-1|TEF-1
Summary
This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified. [provided by RefSeq, May 2010]
Associated Diseases Colorectal Carcinoma
Morphology Adherent
Passage Ratio 1/5-1/4，2days
Complete Culture Medium mcCoy5A+10%FBS
Freezing Medium 90%FBS/Complete culture medium+10% DMSO
QC Indels validated by Sanger sequencing; sterility confirmed via microbial testing.
* For research use only. Not intended for use in humans or animals, including clinical, therapeutic, or diagnostic purposes.
LociSTR Info (Sample Cell)
Sample Cell Line: HCT 116		STR Info (Cell bank)
Cell Line: HCT 116
Allele1Allele2Allele3Allele4Allele1Allele2Allele3Allele4
Amelogenin X X
CSF1PO 7 10 7 9 10 11
D2S1338 16 16
D3S1358 12 17 18 19 12 18 19
D5S818 10 11 10 11
D7S820 11 12 11 12
D8S1179 10 12 14 15 10 12 14 15
D13S317 10 12 10 12
D16S539 11 13 11 12 13 14
D18S51 16 17 16 17
D19S433 12 13 12
D21S11 29 30 29 30
FGA 18 23 18 23
Penta D 9 13 9 13
Penta E 12 13 14 12 13 14
TH01 8 9 8 9
TPOX 8 8
vWA 17 21 22 23 17 21 22 23
D6S1043 13
D12S391 17 21 22
D2S441 11 12
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.
* Research Use Disclaimer: Content is generated from publicly available research data, bioinformatic resources, and computational analyses for research reference only.

Research Publications

IF=2.2
Biochemical and biophysical research communications
N6-methyladenosine (mA) mRNA modification has been defined as a crucial regulator in various biological processes. Recent studies indicated an essential role of YTHDF1, an mA reader, in the maintenance of intestinal stem cells (ISCs), while the detailed mechanism remains to be explored. By searching our mA sequencing, RNA sequencing, and ribosome profiling data, we identified the transcriptional enhanced associate domain 1 (TEAD1) as a direct target of YTHDF1. We confirmed the presence of mA modifications in TEAD1 mRNA and its binding with YTHDF1. Knockdown of either mA methyltransferase METTL3 or YTHDF1 reduced the translation of TEAD1. TEAD1 was highly expressed in ISCs, while depletion of TEAD1 inhibited proliferation and induced differentiation of organoids. Overexpression of TEAD1 reversed the impaired stemness elicited by YTHDF1 depletion. These findings identify TEAD1 as a functional target of mA-YTHDF1 in sustaining intestinal stemness.

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