ACTL7A Knockout HEK293 Cell Line
Cat.No.:
EDJ-KQ7200
Species:
Human
Cell Name:
HEK293
Gene:
ACTL7A
Gene ID:
10881
Size:
1×10⁶cells
ACTL7A Knockout Cell Line (HEK293) is an exclusive upgraded CRISPR/Cas9 system-mediated gene knockout cell, with the advantages of Optimized Strategy Design, Efficient Cell Transfection, High-Performance Cas9 Protein and Hassle-Free Cell Selection.
| Cat.No. | EDJ-KQ7200 |
|---|---|
| Product Name | ACTL7A Knockout Cell Line (HEK293) |
| Cell Line | HEK293 |
| Cellosaurus ID | CVCL_0045 |
| Cell Line Synonyms | Hek293, HEK-293, HEK/293, (HEK)293, HEK 293, HEK,293, 293, 293 HEK, 293 Ad5, Graham 293, Graham-293, Human Embryonic Kidney 293 |
| Gene | |
| NCBI Gene ID | |
| Gene Synonyms | SPGF86 |
| Summary |
The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene (ACTL7A), and related gene, ACTL7B, are intronless, and are located approximately 4 kb apart in a head-to-head orientation within the familial dysautonomia candidate region on 9q31. Based on mutational analysis of the ACTL7A gene in patients with this disorder, it was concluded that it is unlikely to be involved in the pathogenesis of dysautonomia. The ACTL7A gene is expressed in a wide variety of adult tissues, however, its exact function is not known. [provided by RefSeq, Jul 2008]
|
| Associated Diseases | Non-tumor |
| Morphology | Adherent |
| Passage Ratio | 1/5，2days |
| Complete Culture Medium | DMEM + 10% FBS |
| Freezing Medium | 95% Complete culture medium+ 5% DMSO |
| QC | Indels validated by Sanger sequencing; sterility confirmed via microbial testing. |
* For research use only. Not intended for use in humans or animals, including clinical, therapeutic, or diagnostic purposes.
| Loci | STR Info (Sample Cell) Sample Cell Line: HEK293 | STR Info (Cell bank) Cell Line: HEK293 | ||
| Allele1 | Allele2 | Allele1 | Allele2 | |
| Amelogenin | X | X | ||
| CSF1P0 | 12 | 11 | 12 | |
| D2S1338 | 19 | 19 | ||
| D3S1358 | 15 | 17 | 15 | 17 |
| D5S818 | 8 | 8 | 9 | |
| D7S820 | 11 | 12 | 11 | 12 |
| D8S1179 | 12 | 14 | 12 | 14 |
| D13S317 | 12 | 14 | 12 | 14 |
| D16S539 | 9 | 13 | 9 | 13 |
| D18S51 | 17 | 18 | 17 | 18 |
| D19S433 | 15 | 18 | 15 | 18 |
| D21S11 | 28 | 30.2 | 28 | 30.2 |
| FGA | 23 | 23 | ||
| Penta D | 9 | 10 | 9 | 10 |
| Penta E | 7 | 15 | 7 | 15 |
| TH01 | 7 | 9.3 | 7 | 9.3 |
| TPOX | 11 | 11 | ||
| vWA | 16 | 19 | 16 | 19 |
| D6S1043 | 11 | 11 | ||
| D12S391 | 19 | 21 | 11 | 15 |
| D2S441 | 11 | 15 | 11 | 15 |
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.
Conclusion: The STR identification of this cell is correct.
* Research Use Disclaimer: Content is generated from publicly available research data, bioinformatic resources, and computational analyses for research reference only.
Research Publications
Actin-related protein T3 is required for acrosome biogenesis and sperm function in mice.
IF=3.6
Development (Cambridge, England)
Actin-related protein T3 (ACTRT3) is localized in the perinuclear theca (PT) of murine spermatids. We generated Actrt3-/- male mice and showed that they are subfertile, with defects of the acrosome first observed during cap phase. Actrt3 deficiency causes reduced protein levels of the trans-Golgi network markers TGN46 and GOPC and mislocalization of the cis-Golgi protein GM130. Reduction of the autophagy markers LC3B, CTSB and mTOR indicates that loss of ACTRT3 leads to impaired Golgi trafficking and autophagic flux, which are required for acrosome biogenesis. In addition, levels of PFN3, a protein involved in acrosome biogenesis, were significantly reduced. Further, co-immunoprecipitation revealed interaction of ACTRT3 with the PT proteins ACTRT1, ACTRT2, ACTL7A, SPEM2 and the sperm surface protein ZPBP. This suggested that ACTRT3 is a part of the complex 3D scaffold of the PT and contributes to ZPBP localization. Mass spectrometry revealed enrichment of cytoskeletal regulators such as CFL1 and CNN1. Expression of Actrt3 caused changes in HEK239T cell shape and F-actin filament distribution, suggesting a role in cytoskeletal shaping. We conclude that lack of ACTRT3 affects acrosome biogenesis, PT structure and actin remodeling.
This KO model may be useful for:
- Investigating the role of ACTL7A in acrosome biogenesis and sperm function
- Studying actin-related protein signaling pathways in male fertility
- Functional validation of ACTL7A in reproductive biology
- Modeling genetic defects associated with spermatogenesis and infertility
- Supporting drug or target screening for male contraceptive development
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