Description

This kit is based on rapid nucleic acid amplification technology that functions under isothermal conditions at a moderate temperature (typically 39-42 °C). During the reaction, reverse transcriptase synthesizes a complementary DNA (cDNA) strand from the RNA template using specific primers. With the assistance of accessory proteins and single-stranded binding proteins, the recombinase forms a complex with the primers to search for and bind to homologous regions of the target sequence. A D-loop structure is then formed at the homologous site, initiating strand exchange. As the recombinase dissociates from the complex, DNA polymerase binds to the 3' end of the primer and begins strand extension. Detection of the amplification product is achieved through a molecular probe specifically designed based on the target sequence. The probe’s activity depends on the function of nfo enzyme, and the final result is visualized using colloidal gold technology (sandwich immunoassay format).

Kit composition

System Configuration

Reaction Conditions

42 ºC; 8-12 minutes.

Storage

Shipping Temperature: Stable temperature of ≤ 20 ºC.
Storage Conditions: Store at ≤ -20 ºC (±5 ºC) in a constant temperature environment, protected from light, and avoid heavy pressure and repeated freeze-thaw cycles.
Shelf Life: 14 months.
Production Date: See the outer packaging.