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Mouse Subpooled CRISPRi v2 Libraries-Membrane Proteins

Mouse Subpooled CRISPRi v2 Libraries-Membrane Proteins

Cat. No EDCLVS0052-I07
Size
Species Mouse
Library Type CRISPRi

Product Details

Aliases Addgene Catalog # 83994 Prouct Name Mouse Subpooled CRISPRi v2 Libraries-Membrane Proteins
Species Mouse Library Type CRISPRi Library
Plasmid System Two-Vector System Viral Packaging System Third Generation Viral Packaging System
Target Genes 2110 Number Of Sgrnas 11260
Non-targeting Control Sgrna 250 Tag puro
* For research use only. Not intended for use in humans or animals, including clinical, therapeutic, or diagnostic purposes.

Description

EDITGENE specializes in providing high-quality CRISPR screening services. First, a high-coverage and homogeneous plasmid library is prepared (Gini Index <0.1, coverage rate >99%). The plasmid library is then packaged into lentivirus using a well-established system. Virus supernatant is collected, concentrated, and titrated using a cell-based assay to ensure high infection efficiency. The CRISPR library lentivirus provided by EDITGENE can be directly used to construct a library cell pool, streamlining the process by eliminating steps like plasmid library amplification, plasmid library QC, and viral packaging, thus accelerating the experimental workflow.

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