Human SAM Library in lenti sgRNA(MS2)_zeo backbone
Product Details
| Aliases | Addgene Catalog # 1000000057 | Prouct Name | Human Subpooled CRISPRi-v2 Libraries-Cancer and Apoptosis (h2), top 5 sgRNAs/gene |
|---|---|---|---|
| Species | Human | Library Type | CRISPRa Library |
| Plasmid System | Three-Vector System | Viral Packaging System | Third Generation Viral Packaging System |
| Target Genes | 23430 | Number Of Sgrnas | 70290 |
| Tag | zeo |
* For research use only. Not intended for use in humans or animals, including clinical, therapeutic, or diagnostic purposes.
Description
EDITGENE specializes in providing high-quality CRISPR screening services. First, a high-coverage and homogeneous plasmid library is prepared (Gini Index <0.1, coverage rate >99%). The plasmid library is then packaged into lentivirus using a well-established system. Virus supernatant is collected, concentrated, and titrated using a cell-based assay to ensure high infection efficiency. The CRISPR library lentivirus provided by EDITGENE can be directly used to construct a library cell pool, streamlining the process by eliminating steps like plasmid library amplification, plasmid library QC, and viral packaging, thus accelerating the experimental workflow.
Backbone Map
