Human CRISPR Inhibition Pooled Library (Dolcetto)(B)
more
Cat.No: EDCLVS0059-I10-B
Size
1×10^8TU 5×10^8TU 1×10^9TU
Quantity
| Aliases | Addgene Catalog # 1000000114 B-92386 |
|---|---|
| Prouct Name | Human Subpooled CRISPRa-v2 Libraries- Membrane Proteins (h6), top 5 sgRNAs/gene |
| Species | Human |
| Library Type | CRISPRi Library |
| Plasmid System | Two-Vector System |
| Viral Packaging System | Third Generation Viral Packaging System |
| Target Genes | 18899 |
| Number Of Sgrnas | 57011 |
| Non-targeting Control Sgrna | 500 |
| Tag | puro |
Description
EDITGENE specializes in providing high-quality CRISPR screening services. First, a high-coverage and homogeneous plasmid library is prepared (Gini Index <0.1, coverage rate >99%). The plasmid library is then packaged into lentivirus using a well-established system. Virus supernatant is collected, concentrated, and titrated using a cell-based assay to ensure high infection efficiency. The CRISPR library lentivirus provided by EDITGENE can be directly used to construct a library cell pool, streamlining the process by eliminating steps like plasmid library amplification, plasmid library QC, and viral packaging, thus accelerating the experimental workflow.
Backbone Map
