WIF1: Wnt Inhibitory Factor 1
A secreted antagonist of Wnt signaling with roles in development and cancer
Gene Information Card
| Symbol | WIF1 |
|---|---|
| Full Name | WNT inhibitory factor 1 |
| Gene Type | protein-coding |
| Chromosomal Location | 12q14.3 |
| NCBI Gene ID | 11197 ncbi.nlm.nih.gov/gene/11197 |
| Ensembl ID | ENSG00000156076 |
| UniProt ID | Q9Y5W5 |
| OMIM ID | 605186 |
| HGNC ID | 16356 |
| Aliases | WIF-1, WIF1a, WIF1b |
Description
WIF1 encodes a secreted protein that binds to WNT proteins and inhibits their signaling. It acts as a tumor suppressor by antagonizing the canonical WNT/β-catenin pathway. WIF1 is frequently silenced by promoter methylation in various cancers, leading to aberrant WNT activation.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Colorectal cancer | Promoter hypermethylation silences WIF1, leading to constitutive WNT signaling | PMID: 17384680 |
| Lung cancer | Loss of WIF1 expression via methylation contributes to tumor progression | PMID: 15696293 |
| Prostate cancer | WIF1 downregulation correlates with metastasis and poor prognosis | PMID: 20010866 |
| Osteoarthritis | WIF1 polymorphisms associated with cartilage degradation | PMID: 23300811 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Lung | 12.5 | Medium |
| Kidney | 9.8 | Medium |
| Liver | 6.2 | Low |
| Heart | 4.1 | Low |
| Brain | 2.3 | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK 293 | 15.0 | Embryonic kidney cells |
| A549 | 8.5 | Lung carcinoma |
| HCT116 | 3.2 | Colorectal carcinoma |
| MCF7 | 1.1 | Breast cancer |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1A>G | Missense | <0.1% | Unknown |
| c.214C>T | Nonsense | <0.1% | Truncation |
| c.500_501insA | Frameshift | <0.1% | Loss of function |
Mutation functional classification
Loss of Function (LOF)
Promoter methylation and truncating mutations reduce WIF1 expression or activity, leading to unchecked WNT signaling.
Gain of Function (GOF)
Not reported.
Dominant Negative (DN)
Not reported.
View complete mutation data：
Gene Ontology (GO)
| • Wnt-protein binding (GO:0017147) | • negative regulation of Wnt signaling pathway (GO:0030178) |
| • extracellular region (GO:0005576) | • extracellular space (GO:0005615) |
Pathways
• Wnt signaling pathway (KEGG: hsa04310)
• Negative regulation of Wnt signaling (Reactome: R-HSA-4641262)
Protein Summary
WIF1 is a 379-amino-acid secreted glycoprotein containing a WIF domain and five EGF-like domains. It binds directly to WNT ligands, preventing their interaction with Frizzled receptors and thereby inhibiting both canonical and non-canonical WNT signaling. The protein is expressed in multiple tissues and is frequently lost in cancers due to epigenetic silencing.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| WIF1 Knockout HEK293 Cell Line | EDJ-KQ346 | Human | 11197 | Details Get a Quote |
| WIF1 Knockout HeLa Cell Line | EDJ-KQ55600 | Human | 11197 | Details Get a Quote |
| WIF1 Knockout A-549 Cell Line | EDJ-KQ64096 | Human | 11197 | Details Get a Quote |
| WIF1 Knockout HCT 116 Cell Line | EDJ-KQ72546 | Human | 11197 | Details Get a Quote |
