USP25 Gene: Ubiquitin Specific Peptidase 25
A comprehensive biomedical resource on USP25, including genomic data, expression, mutations, and disease associations.
Gene Information Card
| Symbol | USP25 |
|---|---|
| Full Name | Ubiquitin Specific Peptidase 25 |
| Gene Type | Protein coding |
| Chromosomal Location | 21q11.2 |
| NCBI Gene ID | 29761 ncbi.nlm.nih.gov/gene/29761 |
| Ensembl ID | ENSG00000142173 |
| UniProt ID | Q9UHP3 |
| OMIM ID | 604736 |
| HGNC ID | 12625 |
| Aliases | USP21, USP25a, USP25b |
Description
USP25 (Ubiquitin Specific Peptidase 25) is a protein-coding gene that encodes a deubiquitinating enzyme (DUB) belonging to the ubiquitin-specific protease family. The enzyme removes ubiquitin from target proteins, regulating protein stability, localization, and activity. USP25 is involved in immune signaling, inflammation, and cancer. Alternative splicing generates multiple isoforms with distinct functions.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast Cancer | USP25 overexpression stabilizes oncogenic substrates (e.g., ERα, cyclin D1) via deubiquitination, promoting cell proliferation. | PMID: 25609812; COSMIC |
| Colorectal Cancer | USP25 upregulation correlates with poor prognosis; deubiquitinates and stabilizes β-catenin, enhancing Wnt signaling. | PMID: 27323853; COSMIC |
| Inflammatory Bowel Disease | USP25 negatively regulates TLR signaling by deubiquitinating TRAF3, reducing pro-inflammatory cytokine production. | PMID: 25609812; ClinVar |
| Alzheimer's Disease | USP25 modulates tau protein aggregation via deubiquitination, affecting neurodegeneration pathways. | PMID: 30321538; OMIM |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 12.5 | Medium |
| Heart | 8.3 | Low |
| Liver | 15.7 | Medium |
| Lung | 9.1 | Low |
| Kidney | 11.2 | Medium |
| Testis | 22.4 | High |
| Colon | 14.8 | Medium |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 18.5 | High expression |
| HeLa | 12.3 | Medium expression |
| MCF7 | 15.1 | Medium expression |
| HCT116 | 20.7 | High expression |
| A549 | 9.8 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1123C>T (p.R375W) | Missense | <0.01% | Alters substrate binding; potential loss of function |
| c.1567G>A (p.V523M) | Missense | <0.01% | Unknown effect; reported in COSMIC |
| c.2014_2015insA (p.K672fs) | Frameshift | <0.01% | Predicted loss of function via nonsense-mediated decay |
Mutation functional classification
Loss of Function (LOF)
Frameshift and nonsense mutations in USP25 are predicted to cause loss of deubiquitinase activity, impairing regulation of immune signaling and protein turnover.
Gain of Function (GOF)
Missense mutations (e.g., p.R375W) may enhance substrate affinity or alter specificity, potentially leading to oncogenic stabilization of targets.
Dominant Negative (DN)
No dominant-negative mutations have been reported for USP25 in current databases.
View complete mutation data：
Gene Ontology (GO)
| • thiol-dependent deubiquitinase (GO:0004843) | • ubiquitin-dependent protein catabolic process (GO:0006511) |
| • nucleus (GO:0005634) | • cytoplasm (GO:0005737) |
| • proteasome-mediated ubiquitin-dependent protein catabolic process (GO:0043161) |
Pathways
• Ubiquitin-proteasome pathway (KEGG: hsa04120)
• TNF signaling pathway (KEGG: hsa04668)
• Wnt signaling pathway (KEGG: hsa04310)
Protein Summary
USP25 is a 1055-amino acid deubiquitinating enzyme with an N-terminal ubiquitin-associated (UBA) domain and a C-terminal catalytic USP domain. It cleaves ubiquitin chains from target proteins, regulating their stability and function. USP25 is implicated in immune responses (TLR signaling), cell cycle control, and cancer progression. Isoform-specific functions exist due to alternative splicing.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| USP25 Knockout HEK293 Cell Line | EDJ-KQ16075 | Human | 29761 | Details Get a Quote |
| USP25 Knockout HCT 116 Cell Line | EDJ-KQ18080 | Human | 29761 | Details Get a Quote |
| USP25 Knockout A-549 Cell Line | EDJ-KQ47202 | Human | 29761 | Details Get a Quote |
| USP25 Knockout HeLa Cell Line | EDJ-KQ47203 | Human | 29761 | Details Get a Quote |
