UBE2F
Ubiquitin Conjugating Enzyme E2 F (NEDD8 Conjugating Enzyme)
Gene Information Card
| Symbol | UBE2F |
|---|---|
| Full Name | Ubiquitin Conjugating Enzyme E2 F (Putative) |
| Gene Type | Protein coding |
| Chromosomal Location | 2q37.3 |
| NCBI Gene ID | 140739 ncbi.nlm.nih.gov/gene/140739 |
| Ensembl ID | ENSG00000115128 |
| UniProt ID | Q969M7 |
| OMIM ID | 617899 |
| HGNC ID | 12479 |
| Aliases | NCE2, UBC6, UBE2F-S, UBE2F-L |
Description
UBE2F (Ubiquitin Conjugating Enzyme E2 F) encodes a member of the ubiquitin-conjugating enzyme family. This protein specifically conjugates NEDD8, a ubiquitin-like protein, to target proteins such as cullins, thereby regulating cullin-RING ubiquitin ligases (CRLs) and influencing protein degradation, cell cycle progression, and stress responses. UBE2F is implicated in cancer and other diseases.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Lung cancer | UBE2F overexpression promotes NEDD8 conjugation to cullins, enhancing CRL activity and degrading tumor suppressors | COSMIC, literature |
| Colorectal cancer | UBE2F upregulation correlates with poor prognosis; promotes cell proliferation via NEDD8 pathway | COSMIC, literature |
| Hepatocellular carcinoma | UBE2F overexpression linked to increased tumor growth and metastasis | COSMIC, literature |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.5 | Medium |
| Lymph node | 8.2 | Low |
| Spleen | 7.1 | Low |
| Bone marrow | 6.8 | Low |
| Lung | 5.3 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| A549 (lung cancer) | 15.2 | High expression |
| HCT116 (colorectal cancer) | 12.8 | High expression |
| HepG2 (liver cancer) | 11.5 | High expression |
| HEK293 (embryonic kidney) | 8.9 | Moderate expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.337C>T (p.Arg113Trp) | Missense | <0.01% | Unknown functional impact |
| c.463G>A (p.Gly155Ser) | Missense | <0.01% | Unknown functional impact |
| c.1A>G (p.Met1Val) | Missense | <0.01% | Potential loss of start codon |
Mutation functional classification
Loss of Function (LOF)
No confirmed loss-of-function mutations reported in major databases.
Gain of Function (GOF)
Overexpression in cancers suggests potential gain-of-function via increased NEDD8 conjugation.
Dominant Negative (DN)
No dominant-negative mutations reported.
View complete mutation data：
Gene Ontology (GO)
| • NEDD8 conjugating enzyme activity | • ubiquitin-like protein conjugating enzyme activity |
| • protein neddylation | • cullin deneddylation |
| • cell cycle |
Pathways
• NEDD8 conjugation pathway
• Cullin-RING ubiquitin ligase regulation
• Protein ubiquitination
Protein Summary
UBE2F is a 185-amino acid protein (20.9 kDa) that functions as a NEDD8-conjugating enzyme. It transfers NEDD8 from the E1 enzyme to cullin proteins, a process essential for CRL activation. UBE2F is localized to the nucleus and cytoplasm and is overexpressed in several cancers, where it promotes tumor growth by enhancing CRL-mediated degradation of tumor suppressors.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| UBE2F Knockout HEK293 Cell Line | EDJ-KQ3914 | Human | 140739 | Details Get a Quote |
| UBE2F Knockout A-549 Cell Line | EDJ-KQ26137 | Human | 140739 | Details Get a Quote |
| UBE2F Knockout HCT 116 Cell Line | EDJ-KQ26138 | Human | 140739 | Details Get a Quote |
| UBE2F Knockout HeLa Cell Line | EDJ-KQ24795 | Human | 140739 | Details Get a Quote |
