TRMO
tRNA Methyltransferase O (tRNA m5U Methyltransferase)
Gene Information Card
| Symbol | TRMO |
|---|---|
| Full Name | tRNA Methyltransferase O (tRNA m5U Methyltransferase) |
| Gene Type | Protein coding |
| Chromosomal Location | 9q34.11 |
| NCBI Gene ID | 51560 ncbi.nlm.nih.gov/gene/51560 |
| Ensembl ID | ENSG00000136869 |
| UniProt ID | Q9H0K6 |
| OMIM ID | 610166 |
| HGNC ID | 25296 |
| Aliases | TRM5, TRM5L, TRMT5, TRMO1, hTrm5 |
Description
The TRMO gene encodes tRNA methyltransferase O, an enzyme responsible for the 5-methyluridine (m5U) modification at position 54 of tRNAs. This modification is critical for tRNA stability, folding, and translational fidelity. TRMO is a member of the SPOUT methyltransferase family and is conserved across eukaryotes. Mutations and altered expression of TRMO have been implicated in cancer and mitochondrial dysfunction.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Cancer (multiple types) | Altered tRNA modification may affect translation of oncogenes/tumor suppressors | COSMIC mutation data; ClinVar variants |
| Mitochondrial disorders | Defective tRNA methylation impairs mitochondrial protein synthesis | OMIM #610166; functional studies |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.5 | Medium |
| Brain | 8.2 | Low |
| Liver | 6.1 | Low |
| Heart | 5.4 | Low |
| Kidney | 4.8 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 10.3 | Moderate expression |
| HeLa | 8.7 | Moderate expression |
| K562 | 6.2 | Low expression |
| HepG2 | 5.9 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1A>G | Missense | <0.01% | Unknown |
| c.214C>T | Nonsense | <0.01% | Likely loss of function |
| c.347G>A | Missense | <0.01% | Unknown |
Mutation functional classification
Loss of Function (LOF)
Nonsense and frameshift mutations predicted to cause loss of tRNA methylation activity.
Gain of Function (GOF)
Not reported.
Dominant Negative (DN)
Not reported.
View complete mutation data：
Gene Ontology (GO)
| • tRNA methyltransferase activity | • tRNA modification |
| • methylation | • RNA processing |
| • cytoplasm |
Pathways
• tRNA modification in the nucleus and mitochondria
Protein Summary
TRMO is a 45 kDa protein localized to the cytoplasm and mitochondria. It catalyzes the S-adenosylmethionine-dependent methylation of uridine at position 54 of tRNAs, producing 5-methyluridine. The protein contains a conserved SPOUT methyltransferase domain and is essential for proper tRNA structure and function.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| TRMO Knockout HEK293 Cell Line | EDJ-KQ11132 | Human | 51531 | Details Get a Quote |
| TRMO Knockout A-549 Cell Line | EDJ-KQ39121 | Human | 51531 | Details Get a Quote |
| TRMO Knockout HCT 116 Cell Line | EDJ-KQ39122 | Human | 51531 | Details Get a Quote |
| TRMO Knockout HeLa Cell Line | EDJ-KQ39123 | Human | 51531 | Details Get a Quote |
