TRIM65: Tripartite Motif Containing 65
A TRIM family E3 ubiquitin ligase involved in innate immunity and cancer
Gene Information Card
| Symbol | TRIM65 |
|---|---|
| Full Name | Tripartite Motif Containing 65 |
| Gene Type | Protein coding |
| Chromosomal Location | 17q25.1 |
| NCBI Gene ID | 201292 ncbi.nlm.nih.gov/gene/201292 |
| Ensembl ID | ENSG00000141510 |
| UniProt ID | Q6PJ69 |
| OMIM ID | 616605 |
| HGNC ID | 26979 |
| Aliases | FLJ32709, MGC138290 |
Description
TRIM65 (tripartite motif containing 65) is a protein-coding gene that belongs to the TRIM (tripartite motif) family. The encoded protein functions as an E3 ubiquitin ligase, playing roles in innate immune signaling, antiviral responses, and regulation of RNA-binding proteins. TRIM65 is implicated in cancer progression and neurodegenerative diseases through modulation of protein ubiquitination and degradation.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Lung cancer | TRIM65 overexpression promotes tumor growth and metastasis via ubiquitination of tumor suppressors | PMID: 30936463 |
| Hepatocellular carcinoma | TRIM65 enhances cell proliferation and invasion through NF-κB pathway activation | PMID: 31591463 |
| Viral infection | TRIM65 restricts RNA virus replication by targeting viral proteins for degradation | PMID: 28031477 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Lung | 12.5 | Medium |
| Liver | 8.3 | Low |
| Brain | 6.1 | Low |
| Testis | 15.2 | Medium |
| Spleen | 9.8 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| A549 (lung carcinoma) | 14.7 | High expression |
| HepG2 (hepatocellular carcinoma) | 11.2 | Moderate expression |
| HEK293 (embryonic kidney) | 7.5 | Low expression |
| K562 (leukemia) | 5.3 | Very low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1012C>T (p.Arg338Trp) | Missense | 0.001% (gnomAD) | Unknown functional impact |
| c.1456G>A (p.Glu486Lys) | Missense | 0.002% (gnomAD) | Potential loss of ubiquitin ligase activity |
Mutation functional classification
Loss of Function (LOF)
Rare missense variants (e.g., p.Glu486Lys) may impair E3 ligase activity, reducing substrate ubiquitination.
Gain of Function (GOF)
Not well characterized; overexpression in cancer suggests possible oncogenic gain-of-function.
Dominant Negative (DN)
No dominant-negative mutations reported in literature.
View complete mutation data：
Gene Ontology (GO)
| • GO:0005515 - protein binding | • GO:0004842 - ubiquitin-protein transferase activity |
| • GO:0061630 - ubiquitin protein ligase activity | • GO:0046872 - metal ion binding |
| • GO:0045087 - innate immune response | • GO:0016567 - protein ubiquitination |
| • GO:0005737 - cytoplasm |
Pathways
• Ubiquitin mediated proteolysis (KEGG: hsa04120)
• RIG-I-like receptor signaling pathway (KEGG: hsa04622)
• Innate immune system (Reactome: R-HSA-168249)
Protein Summary
TRIM65 is a 493-amino acid protein containing a RING finger domain, B-box type 1 and type 2, and a coiled-coil region characteristic of TRIM family members. It acts as an E3 ubiquitin ligase, catalyzing the transfer of ubiquitin to target proteins, thereby regulating their stability and function. TRIM65 is involved in antiviral immunity by ubiquitinating viral proteins and modulating host RNA-binding proteins. Its dysregulation is associated with several cancers, where it may promote oncogenic signaling.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| TRIM65 Knockout HEK293 Cell Line | EDJ-KQ970 | Human | 201292 | Details Get a Quote |
| TRIM65 Knockout A-549 Cell Line | EDJ-KQ19978 | Human | 201292 | Details Get a Quote |
| TRIM65 Knockout HCT 116 Cell Line | EDJ-KQ19979 | Human | 201292 | Details Get a Quote |
| TRIM65 Knockout HeLa Cell Line | EDJ-KQ19980 | Human | 201292 | Details Get a Quote |
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