TRIM16: Tripartite Motif Containing 16
A RING-finger E3 ubiquitin ligase involved in autophagy, innate immunity, and cancer suppression.
Gene Information Card
| Symbol | TRIM16 |
|---|---|
| Full Name | Tripartite Motif Containing 16 |
| Gene Type | Protein coding |
| Chromosomal Location | 17p11.2 |
| NCBI Gene ID | 10626 ncbi.nlm.nih.gov/gene/10626 |
| Ensembl ID | ENSG00000108556 |
| UniProt ID | O95390 |
| OMIM ID | 606350 |
| HGNC ID | 17240 |
| Aliases | EBBP, RNF29, TRIM16L |
Description
TRIM16 (Tripartite Motif Containing 16) encodes a member of the tripartite motif (TRIM) family. The protein contains a RING-type zinc finger, B-box, and coiled-coil domains, and functions as an E3 ubiquitin ligase. TRIM16 is involved in autophagy regulation, innate immune responses, and acts as a tumor suppressor in several cancers. It is also known as estrogen-responsive B-box protein (EBBP).
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Neuroblastoma | TRIM16 expression is reduced; loss promotes tumor growth and metastasis. | PMID: 23382211 |
| Breast Cancer | TRIM16 suppresses proliferation and invasion via regulation of autophagy. | PMID: 28122350 |
| Lung Cancer | TRIM16 inhibits epithelial-mesenchymal transition and metastasis. | PMID: 30348671 |
| Melanoma | TRIM16 expression correlates with improved survival; promotes autophagy-dependent cell death. | PMID: 31073085 |
| Osteosarcoma | TRIM16 suppresses tumor growth through p53 stabilization. | PMID: 32015547 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Skin | 12.5 | Medium |
| Lung | 8.3 | Medium |
| Breast | 6.1 | Low |
| Brain | 4.2 | Low |
| Liver | 3.8 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| A549 (Lung) | 9.7 | Cancer cell line |
| MCF7 (Breast) | 7.2 | Cancer cell line |
| SH-SY5Y (Neuroblastoma) | 5.4 | Cancer cell line |
| HEK293 (Embryonic kidney) | 6.8 | Immortalized cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.101C>T (p.Pro34Leu) | Missense | <0.01% | Unknown functional effect |
| c.457G>A (p.Glu153Lys) | Missense | <0.01% | Unknown functional effect |
| c.1120_1121insA (p.Thr374Asnfs*5) | Frameshift | <0.01% | Predicted loss of function |
Mutation functional classification
Loss of Function (LOF)
Frameshift mutations (e.g., p.Thr374Asnfs*5) are predicted to cause loss of E3 ligase activity and autophagy regulation.
Gain of Function (GOF)
No gain-of-function mutations reported in COSMIC or ClinVar.
Dominant Negative (DN)
No dominant-negative mutations reported.
View complete mutation data：
Gene Ontology (GO)
Pathways
• Autophagy - organism-specific biosystem (REACT_17015)
• Innate Immune System (REACT_6900)
• Ubiquitin mediated proteolysis (KEGG:04120)
Protein Summary
TRIM16 is a 574-amino acid protein with a molecular weight of approximately 65 kDa. It contains a RING finger domain (residues 25-65) that confers E3 ubiquitin ligase activity, two B-box domains, and a coiled-coil region. The protein localizes to the cytoplasm and cytosol, and is involved in ubiquitination of target proteins to regulate autophagy and innate immune signaling. TRIM16 is also implicated in the suppression of tumor growth and metastasis in multiple cancer types.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| TRIM16 Knockout HEK293 Cell Line | EDJ-KQ50995 | Human | 10626 | Details Get a Quote |
| TRIM16 Knockout HeLa Cell Line | EDJ-KQ55446 | Human | 10626 | Details Get a Quote |
| TRIM16 Knockout A-549 Cell Line | EDJ-KQ63927 | Human | 10626 | Details Get a Quote |
| TRIM16 Knockout HCT 116 Cell Line | EDJ-KQ72384 | Human | 10626 | Details Get a Quote |
