SRPK1: SRSF Protein Kinase 1
A key regulator of alternative splicing in cancer and angiogenesis
Gene Information Card
| Symbol | SRPK1 |
|---|---|
| Full Name | SRSF protein kinase 1 |
| Gene Type | protein-coding |
| Chromosomal Location | 6p21.31 |
| NCBI Gene ID | 6732 ncbi.nlm.nih.gov/gene/6732 |
| Ensembl ID | ENSG00000112658 |
| UniProt ID | Q96SB4 |
| OMIM ID | 602859 |
| HGNC ID | 11309 |
| Aliases | SFRSK1, SRPKIN1, STK17 |
Description
SRPK1 encodes a serine/arginine-rich protein kinase that phosphorylates SR splicing factors, regulating alternative splicing. It plays a critical role in angiogenesis by controlling VEGF-A splicing and is implicated in various cancers, including breast, prostate, and colorectal cancer.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast cancer | Overexpression of SRPK1 promotes oncogenic splicing of VEGF-A and other targets | PMID: 25494299 |
| Prostate cancer | SRPK1 inhibition reduces angiogenic VEGF-Axxx isoform, impairing tumor growth | PMID: 25762719 |
| Colorectal cancer | Elevated SRPK1 correlates with poor prognosis and altered splicing of CD44 | PMID: 28194038 |
| Retinopathy of prematurity | SRPK1 drives pathological angiogenesis via VEGF-A splicing switch | PMID: 23178126 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 28.5 | High |
| Lymph node | 15.2 | Medium |
| Breast | 10.8 | Medium |
| Brain | 6.3 | Low |
| Liver | 4.1 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| MCF7 (breast cancer) | 18.7 | Overexpressed vs normal |
| PC3 (prostate cancer) | 22.1 | High expression |
| HCT116 (colorectal cancer) | 16.4 | Moderate expression |
| HEK293 (embryonic kidney) | 9.2 | Baseline |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1492G>A (p.Glu498Lys) | Missense | <0.1% | Unknown functional impact |
| c.1124C>T (p.Thr375Met) | Missense | <0.1% | Reported in COSMIC |
| c.1960_1961insA | Frameshift | <0.1% | Loss of function predicted |
Mutation functional classification
Loss of Function (LOF)
Rare frameshift and nonsense variants may reduce kinase activity, but no established LOF phenotype.
Gain of Function (GOF)
Overexpression and amplification are more common than activating mutations; no recurrent gain-of-function mutations reported.
Dominant Negative (DN)
No dominant-negative mutations described in literature.
View complete mutation data：
Gene Ontology (GO)
| • GO:0004674 (protein serine/threonine kinase activity) | • GO:0006397 (mRNA processing) |
| • GO:0008380 (RNA splicing) | • GO:0016772 (transferase activity |
| • transferring phosphorus-containing groups) | • GO:0046777 (protein autophosphorylation) |
| • GO:0005737 (cytoplasm) | • GO:0005634 (nucleus) |
Pathways
• Alternative splicing regulation (Reactome: R-HSA-72172)
• VEGF signaling pathway (KEGG: hsa04370)
• SRP-dependent cotranslational protein targeting to membrane (Reactome: R-HSA-1799339)
Protein Summary
SRPK1 is a 655-amino acid serine/threonine kinase that specifically phosphorylates serine/arginine-rich splicing factors (SRSF1, SRSF2). It localizes to both nucleus and cytoplasm and regulates alternative splicing of genes involved in angiogenesis, cell cycle, and apoptosis. SRPK1 is a therapeutic target in cancer and ocular neovascular diseases.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| SRPK1 Knockout HEK293 Cell Line | EDJ-KQ5841 | Human | 6732 | Details Get a Quote |
| SRPK1 Knockout A-549 Cell Line | EDJ-KQ29294 | Human | 6732 | Details Get a Quote |
| SRPK1 Knockout HCT 116 Cell Line | EDJ-KQ29295 | Human | 6732 | Details Get a Quote |
| SRPK1 Knockout HeLa Cell Line | EDJ-KQ29296 | Human | 6732 | Details Get a Quote |
| SRPK1 Knockout HAP1 Cell Line | EDC09530 | Human | 6732 | Details Get a Quote |
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