SPAG5 (Sperm Associated Antigen 5)
A mitotic spindle-associated protein involved in cell cycle regulation and cancer progression
Gene Information Card
| Symbol | SPAG5 |
|---|---|
| Full Name | Sperm Associated Antigen 5 |
| Gene Type | Protein coding |
| Chromosomal Location | 17q11.2 |
| NCBI Gene ID | 10615 ncbi.nlm.nih.gov/gene/10615 |
| Ensembl ID | ENSG00000108298 |
| UniProt ID | O75934 |
| OMIM ID | 609276 |
| HGNC ID | 11214 |
| Aliases | Astrin, MAP126, DEEPEST, hMAP126 |
Description
SPAG5 (Sperm Associated Antigen 5) encodes a mitotic spindle-associated protein that localizes to the spindle poles and kinetochores during mitosis. It is essential for proper chromosome segregation and cell cycle progression. Overexpression is observed in multiple cancers and correlates with poor prognosis.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast cancer | Overexpression promotes proliferation and metastasis | PMID: 29367642 |
| Lung adenocarcinoma | Upregulation associated with poor survival | PMID: 31591465 |
| Hepatocellular carcinoma | SPAG5 silencing reduces tumor growth | PMID: 30737462 |
| Ovarian cancer | High expression linked to chemoresistance | PMID: 32015548 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 48.2 | High |
| Bone marrow | 12.1 | Medium |
| Lymph node | 8.5 | Medium |
| Breast | 4.3 | Low |
| Lung | 3.9 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 15.6 | Cervical cancer cell line |
| MCF7 | 22.3 | Breast cancer cell line |
| A549 | 18.7 | Lung cancer cell line |
| HepG2 | 14.2 | Liver cancer cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1234C>T | Missense | 0.02% | p.Pro412Ser; uncertain significance |
| c.789_790insA | Frameshift | <0.01% | Loss of function |
| c.1567G>A | Synonymous | 0.05% | No effect on protein |
Mutation functional classification
Loss of Function (LOF)
Frameshift mutations leading to premature stop codons are loss-of-function.
Gain of Function (GOF)
Amplification and overexpression in cancers suggest gain-of-function.
Dominant Negative (DN)
Not reported.
View complete mutation data：
Gene Ontology (GO)
| • mitotic spindle organization | • microtubule binding |
| • cell division | • kinetochore assembly |
| • spindle pole body |
Pathways
• Cell Cycle
• Mitotic
• Kinetochore-MT attachment
• Aurora B signaling
Protein Summary
SPAG5 (Astrin) is a 126 kDa protein that forms a complex with kinastrin (SKAP) and localizes to spindle microtubules and kinetochores. It stabilizes microtubule attachments and is required for accurate chromosome segregation. The protein contains a coiled-coil domain and a microtubule-binding region.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| SPAG5 Knockout HEK293 Cell Line | EDJ-KQ15426 | Human | 10615 | Details Get a Quote |
| SPAG5 Knockout A-549 Cell Line | EDJ-KQ46211 | Human | 10615 | Details Get a Quote |
| SPAG5 Knockout HCT 116 Cell Line | EDJ-KQ46212 | Human | 10615 | Details Get a Quote |
| SPAG5 Knockout HeLa Cell Line | EDJ-KQ55443 | Human | 10615 | Details Get a Quote |
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