SLC25A5 (ADP/ATP Translocase 2)
Mitochondrial adenine nucleotide translocator involved in energy metabolism and apoptosis
Gene Information Card
| Symbol | SLC25A5 |
|---|---|
| Full Name | Solute Carrier Family 25 Member 5 |
| Gene Type | Protein coding |
| Chromosomal Location | Xq24 |
| NCBI Gene ID | 292 ncbi.nlm.nih.gov/gene/292 |
| Ensembl ID | ENSG00000137411 |
| UniProt ID | P05141 |
| OMIM ID | 300150 |
| HGNC ID | 10991 |
| Aliases | ANT2, T2, AAC2, ADP/ATP translocase 2 |
Description
SLC25A5 (Solute Carrier Family 25 Member 5) encodes the ADP/ATP translocase 2 (ANT2), a nuclear-encoded mitochondrial inner membrane protein that catalyzes the exchange of ADP and ATP across the mitochondrial membrane. This exchange is essential for oxidative phosphorylation and cellular energy homeostasis. ANT2 also participates in the mitochondrial permeability transition pore (mPTP) and apoptosis regulation. The gene is located on the X chromosome and is widely expressed, with highest levels in tissues with high energy demand.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Cancer (various types) | Overexpression of ANT2 in cancer cells supports glycolytic metabolism and inhibits apoptosis, promoting tumor growth. | PMID: 23455478 |
| Mitochondrial DNA depletion syndrome | Mutations in SLC25A5 can impair mitochondrial nucleotide transport, leading to mtDNA depletion and energy deficiency. | PMID: 27549087 |
| Cardiomyopathy | Dysregulation of ANT2 expression alters mitochondrial ATP/ADP exchange, contributing to cardiac energy failure. | PMID: 25652252 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Heart | 28.5 | High |
| Liver | 22.1 | High |
| Skeletal Muscle | 18.7 | High |
| Brain | 12.3 | Medium |
| Kidney | 10.8 | Medium |
| Lung | 6.4 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 35.2 | High expression |
| HEK293 | 28.9 | High expression |
| HepG2 | 25.6 | High expression |
| K562 | 15.4 | Medium expression |
| A549 | 12.1 | Medium expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.346G>A (p.Gly116Arg) | Missense | <0.01% | Impaired ADP/ATP exchange; associated with mitochondrial disease |
| c.587C>T (p.Thr196Met) | Missense | <0.01% | Reduced protein stability; potential loss of function |
| c.1A>G (p.Met1Val) | Start loss | <0.01% | Loss of translation initiation; likely null allele |
Mutation functional classification
Loss of Function (LOF)
Missense mutations (e.g., p.Gly116Arg) reduce ADP/ATP exchange activity, leading to mitochondrial dysfunction.
Gain of Function (GOF)
Not reported for SLC25A5.
Dominant Negative (DN)
Not reported for SLC25A5.
View complete mutation data：
Gene Ontology (GO)
| • ATP:ADP antiporter activity (GO:0005471) | • integral component of membrane (GO:0016021) |
| • mitochondrial inner membrane (GO:0005743) | • mitochondrial transport (GO:0006839) |
| • ATP transport (GO:0015867) | • ADP transport (GO:0015868) |
| • apoptotic process (GO:0006915) |
Pathways
• KEGG: hsa05012 - Parkinson disease
• KEGG: hsa05016 - Huntington disease
• KEGG: hsa05014 - Amyotrophic lateral sclerosis
• Reactome: R-HSA-1592230 - Mitochondrial calcium ion transport
• Reactome: R-HSA-114608 - Platelet activation
• signaling and aggregation
Protein Summary
The SLC25A5 protein (ANT2) is a 298-amino acid transmembrane protein embedded in the mitochondrial inner membrane. It functions as a homodimer, exchanging cytosolic ADP for mitochondrial ATP with high specificity. ANT2 is a key component of the mitochondrial permeability transition pore (mPTP) and can trigger apoptosis under stress conditions. Its expression is upregulated in many cancers, where it supports the Warburg effect by facilitating ATP export from mitochondria.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| SLC25A5 Knockout HEK293T Cell Line | EDJ-KQ01 | Human | 292 | Details Get a Quote |
| SLC25A52 Knockout HEK293 Cell Line | EDJ-KQ10600 | Human | 147407 | Details Get a Quote |
| SLC25A53 Knockout HEK293 Cell Line | EDJ-KQ11650 | Human | 401612 | Details Get a Quote |
| SLC25A5 Knockout HeLa Cell Line | EDJ-KQ19991 | Human | 292 | Details Get a Quote |
| SLC25A53 Knockout A-549 Cell Line | EDJ-KQ40004 | Human | 401612 | Details Get a Quote |
| SLC25A53 Knockout HCT 116 Cell Line | EDJ-KQ40005 | Human | 401612 | Details Get a Quote |
| SLC25A53 Knockout HeLa Cell Line | EDJ-KQ40006 | Human | 401612 | Details Get a Quote |
| SLC25A5 Knockout A-549 Cell Line | EDJ-KQ21337 | Human | 292 | Details Get a Quote |
| SLC25A5 Knockout HCT 116 Cell Line | EDJ-KQ21338 | Human | 292 | Details Get a Quote |
| SLC25A5 Knockout KYSE-150 Cell Line | EDJ-KZ472 | Human | 292 | Details Get a Quote |
| SLC25A5 Knockout HEK293 Cell Line | EDJ-KQ50123 | Human | 292 | Details Get a Quote |
| SLC25A51 Knockout HEK293 Cell Line | EDC90304 | Human | 92014 | Details Get a Quote |
| SLC25A51 Knockout HeLa Cell Line | EDJ-KQ57822 | Human | 92014 | Details Get a Quote |
| SLC25A52 Knockout HeLa Cell Line | EDJ-KQ58573 | Human | 147407 | Details Get a Quote |
| SLC25A51 Knockout A-549 Cell Line | EDJ-KQ66319 | Human | 92014 | Details Get a Quote |
Displaying Records 1 To 15 Of 18 Records
