SLC22A7 (Solute Carrier Family 22 Member 7)
Organic Anion Transporter 2 (OAT2) - Drug Disposition and Metabolic Homeostasis
Gene Information Card
| Symbol | SLC22A7 |
|---|---|
| Full Name | Solute Carrier Family 22 Member 7 |
| Gene Type | Protein coding |
| Chromosomal Location | 6p21.1 |
| NCBI Gene ID | 10864 ncbi.nlm.nih.gov/gene/10864 |
| Ensembl ID | ENSG00000137266 |
| UniProt ID | Q9Y694 |
| OMIM ID | 604995 |
| HGNC ID | 10972 |
| Aliases | OAT2, NKT |
Description
SLC22A7 encodes the organic anion transporter 2 (OAT2), a transmembrane protein primarily expressed in the liver and kidney. OAT2 mediates the sodium-independent transport of organic anions, including endogenous metabolites (e.g., urate, cyclic nucleotides) and xenobiotics (e.g., methotrexate, antiviral drugs). It plays a critical role in renal and hepatic clearance of drugs and toxins. Genetic variants in SLC22A7 may alter transporter activity and contribute to interindividual variability in drug response and toxicity.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Hyperuricemia | Reduced renal urate excretion due to impaired OAT2 function | PMID: 23395172 |
| Methotrexate toxicity | Decreased hepatic and renal clearance of methotrexate | PMID: 23959308 |
| Chronic kidney disease | Altered OAT2 expression in tubular injury | PMID: 25901030 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Liver | 27.8 | High |
| Kidney | 15.3 | Medium |
| Small intestine | 4.2 | Low |
| Spleen | 1.1 | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HepG2 | 12.5 | Hepatocellular carcinoma cell line |
| HK-2 | 8.9 | Proximal tubular epithelial cell line |
| Caco-2 | 3.4 | Colorectal adenocarcinoma cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.119G>A (p.Arg40His) | Missense | 0.0012 (gnomAD) | Reduced transport activity for methotrexate |
| c.845C>T (p.Thr282Met) | Missense | 0.0008 (gnomAD) | Altered substrate specificity |
| c.1498G>A (p.Val500Ile) | Missense | 0.0005 (gnomAD) | No significant functional change |
Mutation functional classification
Loss of Function (LOF)
p.Arg40His reduces methotrexate uptake by >50% in vitro.
Gain of Function (GOF)
No gain-of-function variants reported in ClinVar or COSMIC.
Dominant Negative (DN)
Not described for SLC22A7.
View complete mutation data：
Gene Ontology (GO)
Pathways
• REACT:15518 - Transport of organic anions
• REACT:111045 - Drug ADME (Absorption
• Distribution
• Metabolism
• Excretion)
• WP:WP1601 - Urate homeostasis
Protein Summary
OAT2 (UniProt Q9Y694) is a 548-amino acid multi-pass membrane protein with 12 transmembrane domains. It functions as an organic anion exchanger, coupling the uptake of organic anions with the efflux of dicarboxylates. The protein is glycosylated and localized to the basolateral membrane of hepatocytes and proximal tubule cells. OAT2 is a key determinant of the pharmacokinetics of many drugs, including antivirals (e.g., acyclovir, ganciclovir) and chemotherapeutics (e.g., methotrexate).
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| SLC22A7 Knockout HEK293 Cell Line | EDJ-KQ51012 | Human | 10864 | Details Get a Quote |
| SLC22A7 Knockout HeLa Cell Line | EDJ-KQ55504 | Human | 10864 | Details Get a Quote |
| SLC22A7 Knockout A-549 Cell Line | EDJ-KQ63993 | Human | 10864 | Details Get a Quote |
| SLC22A7 Knockout HCT 116 Cell Line | EDJ-KQ72444 | Human | 10864 | Details Get a Quote |
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