RPS25: Ribosomal Protein S25
A component of the 40S ribosomal subunit involved in translation initiation and cellular stress responses.
Gene Information Card
| Symbol | RPS25 |
|---|---|
| Full Name | Ribosomal Protein S25 |
| Gene Type | protein-coding |
| Chromosomal Location | 11q23.3 |
| NCBI Gene ID | 6230 ncbi.nlm.nih.gov/gene/6230 |
| Ensembl ID | ENSG00000118181 |
| UniProt ID | P62851 |
| OMIM ID | 180469 |
| HGNC ID | 10413 |
| Aliases | S25, eS25 |
Description
RPS25 encodes ribosomal protein S25, a component of the 40S small ribosomal subunit. This protein is essential for translation initiation, particularly in the recognition and binding of internal ribosome entry sites (IRES) during cap-independent translation. RPS25 is involved in cellular stress responses and has been implicated in ribosomopathies and cancer.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Diamond-Blackfan anemia (DBA) | RPS25 mutations impair ribosome biogenesis, leading to reduced erythroid progenitor cell survival. | ClinVar; PMID: 24675577 |
| Colorectal cancer | RPS25 overexpression promotes IRES-mediated translation of oncogenes, enhancing tumor growth. | COSMIC; PMID: 25772376 |
| Hepatocellular carcinoma | RPS25 upregulation correlates with poor prognosis and increased cell proliferation. | COSMIC; PMID: 29395075 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Liver | 18.5 | High |
| Kidney | 15.2 | High |
| Heart | 12.8 | Medium |
| Brain | 10.1 | Medium |
| Lung | 9.3 | Medium |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 22.1 | High expression |
| HeLa | 19.7 | High expression |
| HepG2 | 17.4 | High expression |
| K562 | 14.6 | Medium expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1A>G (p.Met1?) | Missense | <0.01% | Loss of start codon, likely loss of function |
| c.148C>T (p.Arg50*) | Nonsense | <0.01% | Premature stop, loss of function |
| c.214G>A (p.Gly72Ser) | Missense | 0.02% | Unknown significance |
Mutation functional classification
Loss of Function (LOF)
Nonsense and frameshift mutations in RPS25 are associated with Diamond-Blackfan anemia due to haploinsufficiency.
Gain of Function (GOF)
Not reported.
Dominant Negative (DN)
Not reported.
View complete mutation data：
Gene Ontology (GO)
Pathways
• Eukaryotic Translation Initiation (Reactome: R-HSA-72649)
• Ribosome (KEGG: hsa03010)
• Cap-independent Translation Initiation (Reactome: R-HSA-72737)
Protein Summary
Ribosomal protein S25 (eS25) is a 125-amino-acid protein located in the 40S subunit. It contains a conserved S25 domain and is essential for IRES-mediated translation. The protein interacts with eukaryotic initiation factors and ribosomal RNA to facilitate mRNA binding during translation initiation.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| RPS25 Knockout HEK293 Cell Line | EDJ-KQ50594 | Human | 6230 | Details Get a Quote |
| RPS25 Knockout HeLa Cell Line | EDJ-KQ54363 | Human | 6230 | Details Get a Quote |
| RPS25 Knockout A-549 Cell Line | EDJ-KQ62859 | Human | 6230 | Details Get a Quote |
| RPS25 Knockout HCT 116 Cell Line | EDJ-KQ71324 | Human | 6230 | Details Get a Quote |
