RPS10-NUDT3 gene: readthrough transcript, NUDT3, fusion protein

RPS10-NUDT3 is a readthrough gene located on chromosome 6p21.31. It produces a naturally occurring readthrough transcript that fuses exons from the upstream RPS10 gene (ribosomal protein S10) with the downstream NUDT3 gene (nudix hydrolase 3). The resulting mRNA is translated into a protein that is essentially identical to NUDT3, as the RPS10 portion is not translated due to a stop codon in the readthrough junction. NUDT3 encodes a Nudix hydrolase that cleaves diphosphate bonds in substrates such as diphosphoinositol polyphosphates and mRNA cap analogs, playing roles in mRNA decapping and phosphate metabolism.

Tissue Expression

Cell Line Expression

Hotspot Mutations

Mutation functional classification

• mRNA decapping (Reactome: R-HSA-429958)

• Purine metabolism (KEGG: hsa00230)

The RPS10-NUDT3 readthrough transcript produces a protein that is functionally identical to NUDT3 (UniProt O95989). NUDT3 is a 172-amino acid Nudix hydrolase that catalyzes the hydrolysis of diphosphate bonds in various substrates, including diphosphoinositol polyphosphates and mRNA cap analogs (e.g., m7GpppN). It is involved in mRNA decapping and phosphate metabolism. The protein contains a Nudix box motif (GX5EX7REUXEEXGU, where U is a hydrophobic residue) essential for its catalytic activity. It is localized in the cytoplasm and cytosol.