RNF25: Ring Finger Protein 25 - E3 Ubiquitin Ligase and NF-κB Regulator
Comprehensive genomic and proteomic analysis of RNF25, a RING-type E3 ubiquitin ligase involved in DNA damage response and transcriptional regulation.
Gene Information Card
| Symbol | RNF25 |
|---|---|
| Full Name | ring finger protein 25 |
| Gene Type | protein-coding |
| Chromosomal Location | 2q35 |
| NCBI Gene ID | 64320 ncbi.nlm.nih.gov/gene/64320 |
| Ensembl ID | ENSG00000163581 |
| UniProt ID | Q96TC7 |
| OMIM ID | 617849 |
| HGNC ID | 18098 |
| Aliases | AO7, RNF25_HUMAN |
Description
RNF25 (ring finger protein 25) encodes a RING-type E3 ubiquitin ligase that catalyzes ubiquitination of target proteins, playing a role in the NF-κB signaling pathway and DNA damage response. The protein contains a RING finger domain and a coiled-coil region, and is involved in the regulation of transcription and protein stability.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Cancer (various types) | RNF25 mutations may alter ubiquitination of NF-κB pathway components, affecting cell survival and proliferation. | COSMIC database reports somatic mutations in multiple cancer types. |
| No specific Mendelian disease association | Currently no disease is directly linked to RNF25 in OMIM. | OMIM entry 617849 notes limited clinical data. |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.5 | Medium |
| Brain | 8.3 | Low |
| Lung | 6.1 | Low |
| Liver | 5.4 | Low |
| Kidney | 7.2 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK 293 | 10.2 | Embryonic kidney cells |
| HeLa | 8.9 | Cervical cancer cells |
| A549 | 7.5 | Lung carcinoma cells |
| K562 | 6.8 | Leukemia cells |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.497G>A (p.Arg166Gln) | Missense | 0.01% | Unknown functional effect |
| c.1042C>T (p.Arg348Trp) | Missense | 0.005% | Unknown functional effect |
| c.1234_1235insA (p.Thr412Asnfs*5) | Frameshift | Rare | Predicted loss of function |
Mutation functional classification
Loss of Function (LOF)
Frameshift mutations (e.g., p.Thr412Asnfs*5) likely cause loss of E3 ligase activity.
Gain of Function (GOF)
No gain-of-function mutations reported.
Dominant Negative (DN)
No dominant-negative mutations reported.
View complete mutation data：
Gene Ontology (GO)
Pathways
• Ubiquitin mediated proteolysis (KEGG: hsa04120)
• NF-kappa B signaling pathway (KEGG: hsa04064)
Protein Summary
RNF25 is a 433-amino acid RING finger protein that functions as an E3 ubiquitin ligase. It contains a RING-type zinc finger domain (residues 55-95) and a coiled-coil region (residues 150-200). The protein localizes to the nucleus and cytoplasm, and is involved in ubiquitination of substrates such as NF-κB pathway components, thereby modulating transcriptional responses to stress and DNA damage.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| RNF25 Knockout HEK293 Cell Line | EDJ-KQ2670 | Human | 64320 | Details Get a Quote |
| RNF25 Knockout A-549 Cell Line | EDJ-KQ22088 | Human | 64320 | Details Get a Quote |
| RNF25 Knockout HCT 116 Cell Line | EDJ-KQ23459 | Human | 64320 | Details Get a Quote |
| RNF25 Knockout HeLa Cell Line | EDJ-KQ23460 | Human | 64320 | Details Get a Quote |
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