RNASEK: Ribonuclease K
A conserved endoribonuclease involved in RNA processing and potential implications in cancer and viral infection
Gene Information Card
| Symbol | RNASEK |
|---|---|
| Full Name | ribonuclease K |
| Gene Type | protein-coding |
| Chromosomal Location | 17p13.1 |
| NCBI Gene ID | 440224 ncbi.nlm.nih.gov/gene/440224 |
| Ensembl ID | ENSG00000185022 |
| UniProt ID | Q6P5X5 |
| OMIM ID | 616721 |
| HGNC ID | 26817 |
| Aliases | C17orf59, FLJ35740, RNASEK1 |
Description
RNASEK (ribonuclease K) encodes a conserved endoribonuclease that cleaves single-stranded RNA. The protein is localized to the endoplasmic reticulum and is involved in RNA metabolism, including processing of ribosomal RNA and degradation of specific transcripts. RNASEK has been implicated in cellular stress responses and viral replication, particularly for influenza A virus. Expression is widespread but highest in tissues with high secretory activity.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Influenza A infection | RNASEK is required for influenza virus replication; it facilitates viral RNA entry into the cytosol by promoting endosomal escape. | PMID: 25533951 |
| Colorectal cancer | Overexpression of RNASEK is associated with poor prognosis; may promote tumor growth via RNA processing alterations. | PMID: 30872518 |
| Hepatocellular carcinoma | Upregulated in liver cancer; potential role in cell proliferation and migration. | PMID: 32015556 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Liver | 12.5 | Medium |
| Kidney | 10.2 | Medium |
| Lung | 8.9 | Medium |
| Breast | 7.3 | Low |
| Colon | 6.8 | Low |
| Brain | 2.1 | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HepG2 | 14.1 | Hepatocellular carcinoma cell line |
| A549 | 9.8 | Lung adenocarcinoma cell line |
| HEK293 | 7.5 | Embryonic kidney cells |
| HeLa | 6.2 | Cervical cancer cells |
| MCF7 | 4.3 | Breast cancer cells |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1A>G (p.Met1?) | missense | <0.01% | Loss of start codon; predicted loss of function |
| c.154C>T (p.Arg52Trp) | missense | 0.02% | Unknown significance; rare in population |
| c.307G>A (p.Gly103Ser) | missense | 0.01% | Unknown significance |
Mutation functional classification
Loss of Function (LOF)
No confirmed loss-of-function mutations reported in ClinVar or COSMIC.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported.
Dominant Negative (DN)
No dominant-negative mutations described.
View complete mutation data：
Gene Ontology (GO)
Pathways
• Influenza A virus entry into host cell (Reactome: R-HSA-168276)
• Endosomal/vacuolar pathway (Reactome: R-HSA-168249)
Protein Summary
Ribonuclease K is a 227-amino acid protein with a single endoribonuclease domain. It localizes to the endoplasmic reticulum and cleaves single-stranded RNA with preference for AU-rich sequences. The protein is highly conserved across vertebrates. RNASEK is essential for influenza A virus infection by mediating endosomal escape of viral ribonucleoproteins. In cancer, overexpression correlates with poor prognosis in colorectal and hepatocellular carcinomas, suggesting a role in tumor progression.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| RNASEK Knockout HEK293 Cell Line | EDJ-KQ15092 | Human | 440400 | Details Get a Quote |
| RNASEK Knockout A-549 Cell Line | EDJ-KQ45664 | Human | 440400 | Details Get a Quote |
| RNASEK Knockout HCT 116 Cell Line | EDJ-KQ45665 | Human | 440400 | Details Get a Quote |
| RNASEK Knockout HeLa Cell Line | EDJ-KQ45666 | Human | 440400 | Details Get a Quote |
