RHOF (Ras Homolog Family Member F) Gene
A Rho GTPase Involved in Cytoskeletal Dynamics and Cell Migration
Gene Information Card
| Symbol | RHOF |
|---|---|
| Full Name | Ras homolog family member F |
| Gene Type | Protein coding |
| Chromosomal Location | 12q24.31 |
| NCBI Gene ID | 54509 ncbi.nlm.nih.gov/gene/54509 |
| Ensembl ID | ENSG00000139679 |
| UniProt ID | Q9HBH0 |
| OMIM ID | 609200 |
| HGNC ID | 17368 |
| Aliases | ARHF, RIF, RhoF, MGC39923 |
Description
RHOF (Ras homolog family member F) encodes a member of the Rho family of small GTPases, which regulate cytoskeletal organization, cell adhesion, and migration. RHOF is involved in filopodia formation and neurite outgrowth. It is expressed in various tissues and has been implicated in cancer progression and immune cell function.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast cancer | RHOF overexpression promotes cell migration and invasion via cytoskeletal remodeling | NCBI Gene, COSMIC |
| Colorectal cancer | RHOF upregulation associated with poor prognosis and metastatic potential | NCBI Gene, COSMIC |
| Lung cancer | RHOF expression correlates with tumor stage and lymph node metastasis | NCBI Gene, COSMIC |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 15.2 | Low |
| Lung | 22.8 | Medium |
| Breast | 18.5 | Low |
| Colon | 25.1 | Medium |
| Blood | 12.3 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| MCF7 (breast cancer) | 28.4 | Moderate expression |
| A549 (lung cancer) | 32.1 | Moderate expression |
| HCT116 (colorectal cancer) | 35.7 | Moderate expression |
| HEK293 (embryonic kidney) | 20.5 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.83G>A (p.Arg28His) | Missense | <0.1% | Unknown functional impact |
| c.214C>T (p.Arg72Trp) | Missense | <0.1% | Unknown functional impact |
| c.365A>G (p.Asn122Ser) | Missense | <0.1% | Unknown functional impact |
Mutation functional classification
Loss of Function (LOF)
No confirmed loss-of-function mutations reported in major databases.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported; overexpression observed in cancers.
Dominant Negative (DN)
No dominant-negative mutations described.
View complete mutation data：
Gene Ontology (GO)
| • GTPase activity | • GTP binding |
| • Protein binding | • Actin cytoskeleton organization |
| • Cell migration | • Filopodium assembly |
| • Signal transduction |
Pathways
• Rho GTPase cycle
• Signaling by Rho family GTPases
• RAC1 GTPase cycle
• CDC42 GTPase cycle
Protein Summary
RHOF is a 220-amino acid protein belonging to the Rho GTPase subfamily. It localizes to the plasma membrane and endomembranes, where it cycles between an active GTP-bound and inactive GDP-bound state. RHOF regulates actin dynamics, promoting filopodia formation and cell motility. It is widely expressed and has been linked to cancer metastasis and neuronal development.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| RHOF Knockout HEK293 Cell Line | EDJ-KQ10778 | Human | 54509 | Details Get a Quote |
| RHOF Knockout A-549 Cell Line | EDJ-KQ39736 | Human | 54509 | Details Get a Quote |
| RHOF Knockout HCT 116 Cell Line | EDJ-KQ39738 | Human | 54509 | Details Get a Quote |
| RHOF Knockout HeLa Cell Line | EDJ-KQ39739 | Human | 54509 | Details Get a Quote |
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