RCHY1
Ring Finger and CHY Zinc Finger Domain Containing 1
Gene Information Card
| Symbol | RCHY1 |
|---|---|
| Full Name | Ring Finger and CHY Zinc Finger Domain Containing 1 |
| Gene Type | protein-coding |
| Chromosomal Location | 4q21.1 |
| NCBI Gene ID | 25898 ncbi.nlm.nih.gov/gene/25898 |
| Ensembl ID | ENSG00000163738 |
| UniProt ID | Q96PM5 |
| OMIM ID | 607680 |
| HGNC ID | 17481 |
| Aliases | PIRH2, CHIMP, ZNF363, RNF199, ARNIP |
Description
RCHY1 encodes an E3 ubiquitin-protein ligase that targets p53 for proteasomal degradation, thereby negatively regulating p53-mediated apoptosis and cell cycle arrest. It contains a RING finger domain and a CHY zinc finger domain, and is involved in cellular stress responses, DNA damage repair, and tumorigenesis.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Hepatocellular carcinoma | Overexpression of RCHY1 promotes p53 degradation, reducing tumor suppression | PMID: 15601826 |
| Colorectal cancer | RCHY1 upregulation correlates with poor prognosis and p53 inactivation | PMID: 19029980 |
| Prostate cancer | RCHY1 overexpression enhances androgen receptor signaling and tumor growth | PMID: 16951152 |
| Lung cancer | RCHY1 amplification leads to p53 destabilization and chemoresistance | PMID: 19723653 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.5 | Medium |
| Liver | 8.3 | Medium |
| Kidney | 6.7 | Medium |
| Lung | 5.1 | Low |
| Brain | 2.3 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HepG2 | 10.2 | Hepatocellular carcinoma cell line |
| A549 | 7.8 | Lung adenocarcinoma cell line |
| MCF7 | 4.5 | Breast cancer cell line |
| HEK293 | 3.1 | Embryonic kidney cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1A>G | Missense | <0.01% | p.Met1Val; potential loss of start codon |
| c.100C>T | Nonsense | <0.01% | p.Arg34*; premature truncation |
| c.200_201insA | Frameshift | <0.01% | p.Gln67fs; loss of function |
Mutation functional classification
Loss of Function (LOF)
Nonsense and frameshift mutations that truncate the protein, impairing ubiquitin ligase activity.
Gain of Function (GOF)
Not well documented; overexpression in tumors may act as a gain-of-function by enhancing p53 degradation.
Dominant Negative (DN)
Not reported.
View complete mutation data：
Gene Ontology (GO)
| • ubiquitin-protein transferase activity | • zinc ion binding |
| • protein ubiquitination | • negative regulation of apoptotic process |
| • regulation of cell cycle |
Pathways
• p53 signaling pathway
• Ubiquitin mediated proteolysis
Protein Summary
RCHY1 (PIRH2) is a 261-amino acid E3 ubiquitin ligase that catalyzes the ubiquitination and subsequent proteasomal degradation of p53. It also interacts with other substrates such as androgen receptor and p73, modulating cell proliferation and survival. The protein is overexpressed in several cancers, contributing to tumor progression and therapy resistance.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| RCHY1 Knockout HEK293 Cell Line | EDJ-KQ8289 | Human | 25898 | Details Get a Quote |
| RCHY1 Knockout A-549 Cell Line | EDJ-KQ34246 | Human | 25898 | Details Get a Quote |
| RCHY1 Knockout HCT 116 Cell Line | EDJ-KQ34247 | Human | 25898 | Details Get a Quote |
| RCHY1 Knockout HeLa Cell Line | EDJ-KQ34248 | Human | 25898 | Details Get a Quote |
