PRSS2: Serine Protease 2 (Trypsinogen-2) – Gene Information and Clinical Significance
Comprehensive overview of PRSS2, a key pancreatic trypsinogen gene implicated in pancreatitis and cancer.
Gene Information Card
| Symbol | PRSS2 |
|---|---|
| Full Name | Serine Protease 2 |
| Gene Type | protein-coding |
| Chromosomal Location | 7q34 |
| NCBI Gene ID | 5645 ncbi.nlm.nih.gov/gene/5645 |
| Ensembl ID | ENSG00000125870 |
| UniProt ID | P07478 |
| OMIM ID | 601564 |
| HGNC ID | 9483 |
| Aliases | TRY2, TRY8, TRYP2, trypsinogen-2 |
Description
PRSS2 encodes trypsinogen-2, a serine protease precursor synthesized in the pancreas. It is secreted into the duodenum, where it is activated to trypsin-2, a key digestive enzyme that cleaves peptide bonds at lysine and arginine residues. PRSS2 is part of the trypsinogen family and plays a critical role in protein digestion. Mutations in PRSS2 have been associated with chronic pancreatitis and altered risk for pancreatic cancer.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Chronic Pancreatitis | Loss-of-function variants (e.g., p.Gly191Arg) reduce trypsin activity, protecting against premature trypsin activation and pancreatitis. Gain-of-function variants (e.g., p.Asp22Ala) increase autoactivation risk. | ClinVar, OMIM |
| Pancreatic Cancer | Somatic mutations and altered expression of PRSS2 may contribute to tumor progression. COSMIC reports missense mutations in pancreatic adenocarcinoma. | COSMIC, NCBI |
| Hereditary Pancreatitis | Rare PRSS2 variants (e.g., p.Arg122His) have been linked to familial forms of pancreatitis. | OMIM, ClinVar |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Pancreas | 1234.5 | High |
| Salivary Gland | 12.3 | Low |
| Stomach | 8.7 | Low |
| Duodenum | 5.4 | Low |
| Liver | 1.2 | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| PANC-1 | 456.7 | Pancreatic cancer cell line |
| MIA PaCa-2 | 389.2 | Pancreatic cancer cell line |
| BxPC-3 | 512.3 | Pancreatic cancer cell line |
| HeLa | 2.1 | Cervical cancer cell line (low expression) |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| p.Gly191Arg (c.571G>A) | Missense | 0.5% in general population | Loss-of-function; protective against chronic pancreatitis |
| p.Asp22Ala (c.65A>C) | Missense | <0.1% | Gain-of-function; increased trypsin autoactivation, risk factor for pancreatitis |
| p.Arg122His (c.365G>A) | Missense | <0.1% | Associated with hereditary pancreatitis |
| c.−28A>G | Promoter variant | 1-2% | May alter expression levels; uncertain significance |
Mutation functional classification
Loss of Function (LOF)
p.Gly191Arg reduces catalytic activity, decreasing trypsinogen activation and protecting against pancreatitis.
Gain of Function (GOF)
p.Asp22Ala enhances autoactivation, predisposing to premature trypsin activation and pancreatitis.
Dominant Negative (DN)
No well-characterized dominant-negative mutations reported for PRSS2.
View complete mutation data：
Gene Ontology (GO)
| • serine-type endopeptidase activity (GO:0004252) | • proteolysis (GO:0006508) |
| • extracellular region (GO:0005576) | • extracellular matrix (GO:0031012) |
| • hydrolase activity (GO:0016787) |
Pathways
• Digestion and absorption (Reactome: R-HSA-8963748)
• Trypsin activation cascade (Reactome: R-HSA-8963749)
• Pancreatic secretion (KEGG: hsa04972)
Protein Summary
PRSS2 encodes trypsinogen-2 (cationic trypsinogen), a 247-amino-acid zymogen. After secretion into the duodenum, it is cleaved by enteropeptidase to active trypsin-2. The protein contains a signal peptide (residues 1-15), an activation peptide (16-23), and a catalytic triad (His63, Asp108, Ser203). Trypsin-2 is a serine protease with broad substrate specificity, involved in digestion and extracellular matrix remodeling. Mutations affecting its activation or activity are linked to pancreatic disorders.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| PRSS23 Knockout HEK293 Cell Line | EDJ-KQ2267 | Human | 11098 | Details Get a Quote |
| PRSS2 Knockout HEK293 Cell Line | EDJ-KQ5553 | Human | 5645 | Details Get a Quote |
| PRSS27 Knockout HEK293 Cell Line | EDJ-KQ9920 | Human | 83886 | Details Get a Quote |
| PRSS21 Knockout HEK293 Cell Line | EDJ-KQ14930 | Human | 10942 | Details Get a Quote |
| PRSS22 Knockout HEK293 Cell Line | EDJ-KQ14931 | Human | 64063 | Details Get a Quote |
| PRSS22 Knockout HCT 116 Cell Line | EDJ-KQ44121 | Human | 64063 | Details Get a Quote |
| PRSS23 Knockout A-549 Cell Line | EDJ-KQ22604 | Human | 11098 | Details Get a Quote |
| PRSS23 Knockout HCT 116 Cell Line | EDJ-KQ22605 | Human | 11098 | Details Get a Quote |
| PRSS23 Knockout HeLa Cell Line | EDJ-KQ22606 | Human | 11098 | Details Get a Quote |
| PRSS21 Knockout HeLa Cell Line | EDJ-KQ45380 | Human | 10942 | Details Get a Quote |
| PRSS2 Knockout HeLa Cell Line | EDJ-KQ54228 | Human | 5645 | Details Get a Quote |
| PRSS22 Knockout HeLa Cell Line | EDJ-KQ57012 | Human | 64063 | Details Get a Quote |
| PRSS27 Knockout HeLa Cell Line | EDJ-KQ57495 | Human | 83886 | Details Get a Quote |
| PRSS2 Knockout A-549 Cell Line | EDJ-KQ62721 | Human | 5645 | Details Get a Quote |
| PRSS21 Knockout A-549 Cell Line | EDJ-KQ64024 | Human | 10942 | Details Get a Quote |
