PKN3: Protein Kinase N3 – A Key Regulator in Cell Growth and Cancer
Comprehensive gene card for PKN3, including genomic data, expression, mutations, and disease associations.
Gene Information Card
| Symbol | PKN3 |
|---|---|
| Full Name | Protein Kinase N3 |
| Gene Type | protein-coding |
| Chromosomal Location | 9q34.11 |
| NCBI Gene ID | 29941 ncbi.nlm.nih.gov/gene/29941 |
| Ensembl ID | ENSG00000107165 |
| UniProt ID | Q6P5Z2 |
| OMIM ID | 609446 |
| HGNC ID | 17798 |
| Aliases | PRK3, PKN3, PRKCL3 |
Description
PKN3 (Protein Kinase N3) is a member of the protein kinase C (PKC) superfamily and encodes a serine/threonine kinase involved in cell proliferation, migration, and cytoskeletal organization. It is activated by Rho family small GTPases and plays a role in cancer progression, particularly in prostate and breast cancers. PKN3 is also implicated in insulin signaling and vascular development.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Prostate Cancer | PKN3 overexpression promotes tumor cell invasion and metastasis via Rho/ROCK signaling | PMID: 21715324 |
| Breast Cancer | PKN3 knockdown reduces cell migration and invasion in triple-negative breast cancer cell lines | PMID: 25605247 |
| Insulin Resistance | PKN3 modulates insulin-stimulated glucose uptake in adipocytes | PMID: 19074966 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Prostate | 12.5 | Medium |
| Breast | 8.3 | Low |
| Adipose | 6.7 | Low |
| Lung | 5.1 | Low |
| Brain | 2.3 | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| PC-3 (prostate cancer) | 15.2 | High expression |
| MDA-MB-231 (breast cancer) | 10.8 | Moderate expression |
| HeLa (cervical cancer) | 7.4 | Low expression |
| HUVEC (endothelial) | 4.1 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1246G>A (p.Gly416Arg) | Missense | <0.1% | Unknown functional effect |
| c.187C>T (p.Arg63Trp) | Missense | <0.1% | Reported in COSMIC as likely passenger |
| c.1021_1023del (p.Leu341del) | In-frame deletion | <0.1% | Loss of kinase activity predicted |
Mutation functional classification
Loss of Function (LOF)
In-frame deletions in the kinase domain (e.g., p.Leu341del) are predicted to impair catalytic activity.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported in PKN3.
Dominant Negative (DN)
No dominant-negative variants documented for PKN3.
View complete mutation data：
Gene Ontology (GO)
Pathways
• Rho GTPase cycle (R-HSA-194840)
• Signaling by Rho family GTPases (R-HSA-194315)
• Regulation of actin cytoskeleton (KEGG:04810)
• PI3K-Akt signaling pathway (KEGG:04151)
Protein Summary
PKN3 is a 889-amino-acid serine/threonine kinase with an N-terminal regulatory region containing a Rho-binding domain and a C-terminal catalytic kinase domain. It is activated by binding to RhoA, RhoB, or RhoC, leading to autophosphorylation and subsequent phosphorylation of downstream targets such as vimentin and myosin light chain. PKN3 localizes to the cytoplasm and cell membrane and is involved in cytoskeletal remodeling, cell motility, and tumor invasion.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| PKN3 Knockout HEK293 Cell Line | EDJ-KQ850 | Human | 29941 | Details Get a Quote |
| PKN3 Knockout A-549 Cell Line | EDJ-KQ19641 | Human | 29941 | Details Get a Quote |
| PKN3 Knockout HCT 116 Cell Line | EDJ-KQ19642 | Human | 29941 | Details Get a Quote |
| PKN3 Knockout HeLa Cell Line | EDJ-KQ19643 | Human | 29941 | Details Get a Quote |
| PKN3 Knockout HAP1 Cell Line | EDC08033 | Human | 29941 | Details Get a Quote |
