PKN1: Protein Kinase N1 – A Key Regulator of Cell Signaling and Disease
Comprehensive genomic and proteomic overview of PKN1, including expression, mutations, and clinical relevance.
Gene Information Card
| Symbol | PKN1 |
|---|---|
| Full Name | Protein Kinase N1 |
| Gene Type | protein-coding |
| Chromosomal Location | 19p13.12 |
| NCBI Gene ID | 5585 ncbi.nlm.nih.gov/gene/5585 |
| Ensembl ID | ENSG00000123143 |
| UniProt ID | Q16512 |
| OMIM ID | 601032 |
| HGNC ID | 9405 |
| Aliases | PAK1, PKN, PRK1, PRKCL1 |
Description
PKN1 (Protein Kinase N1) is a serine/threonine-protein kinase that acts as a downstream effector of the small GTPase Rho. It is involved in various cellular processes including cytoskeletal reorganization, cell adhesion, cell migration, and transcriptional regulation. PKN1 is implicated in cancer progression, neurodegenerative disorders, and cardiovascular diseases.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Prostate Cancer | PKN1 overexpression promotes androgen receptor signaling and cell proliferation | PMID: 19029980 |
| Alzheimer Disease | PKN1 phosphorylates tau protein, contributing to neurofibrillary tangle formation | PMID: 15314157 |
| Cardiac Hypertrophy | PKN1 mediates RhoA-induced hypertrophic responses in cardiomyocytes | PMID: 15611087 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 12.3 | Medium |
| Heart | 8.7 | Low |
| Prostate | 15.2 | High |
| Lung | 6.4 | Low |
| Liver | 4.1 | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 10.5 | Cervical cancer cell line |
| PC-3 | 18.2 | Prostate cancer cell line |
| SH-SY5Y | 14.0 | Neuroblastoma cell line |
| MCF-7 | 7.8 | Breast cancer cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1234C>T (p.Arg412Trp) | Missense | 0.02% | Altered kinase activity |
| c.567_568insA (p.Glu190fs) | Frameshift | <0.01% | Loss of function |
| c.2101G>A (p.Glu701Lys) | Missense | 0.01% | Unknown |
Mutation functional classification
Loss of Function (LOF)
Frameshift mutations (e.g., p.Glu190fs) lead to truncated, non-functional PKN1 protein.
Gain of Function (GOF)
Missense mutations (e.g., p.Arg412Trp) may enhance kinase activity, promoting oncogenic signaling.
Dominant Negative (DN)
No well-characterized dominant-negative mutations reported in PKN1.
View complete mutation data：
Gene Ontology (GO)
| • protein serine/threonine kinase activity | • Rho GTPase binding |
| • cytoskeleton organization | • cell migration |
| • apoptotic process | • signal transduction |
Pathways
• Rho GTPase signaling
• Androgen receptor signaling
• Tau phosphorylation in Alzheimer disease
• Cardiac hypertrophy signaling
Protein Summary
PKN1 is a 942-amino acid serine/threonine kinase with an N-terminal regulatory region containing three Rho-binding domains and a C-terminal catalytic kinase domain. It is activated by RhoA binding and proteolytic cleavage. PKN1 phosphorylates substrates such as tau, vimentin, and androgen receptor, influencing cell structure, transcription, and survival.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| PKN1 Knockout HEK293 Cell Line | EDJ-KQ847 | Human | 5585 | Details Get a Quote |
| PKN1 Knockout A-549 Cell Line | EDJ-KQ19636 | Human | 5585 | Details Get a Quote |
| PKN1 Knockout HCT 116 Cell Line | EDJ-KQ19637 | Human | 5585 | Details Get a Quote |
| PKN1 Knockout HeLa Cell Line | EDJ-KQ19638 | Human | 5585 | Details Get a Quote |
