PCLAF (PCNA Clamp Associated Factor)
A key regulator of DNA replication and repair, implicated in cancer progression
Gene Information Card
| Symbol | PCLAF |
|---|---|
| Full Name | PCNA clamp associated factor |
| Gene Type | protein-coding |
| Chromosomal Location | 15q22.31 |
| NCBI Gene ID | 9768 ncbi.nlm.nih.gov/gene/9768 |
| Ensembl ID | ENSG00000137807 |
| UniProt ID | Q15004 |
| OMIM ID | 609699 |
| HGNC ID | 28983 |
| Aliases | KIAA0101, L5, NS5ATP9, OEATC, p15(PAF), PAF |
Description
PCLAF (PCNA clamp associated factor) encodes a protein that binds to proliferating cell nuclear antigen (PCNA) and regulates DNA replication and repair. It is involved in cell cycle progression, DNA damage response, and chromatin remodeling. Overexpression is observed in multiple cancers and correlates with poor prognosis.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Hepatocellular carcinoma | Overexpression promotes cell proliferation and inhibits apoptosis via PCNA interaction | PMID: 25642768 |
| Breast cancer | High expression associated with aggressive subtypes and poor survival | PMID: 29187738 |
| Colorectal cancer | Upregulation enhances DNA repair and chemoresistance | PMID: 30093503 |
| Lung cancer | PCLAF knockdown reduces tumor growth in vitro and in vivo | PMID: 31073040 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.3 | High |
| Bone marrow | 8.7 | Medium |
| Lymph node | 6.5 | Medium |
| Brain | 1.2 | Low |
| Heart | 0.8 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 15.2 | Cervical cancer cell line |
| A549 | 11.4 | Lung adenocarcinoma |
| MCF7 | 9.8 | Breast cancer |
| HepG2 | 14.1 | Hepatocellular carcinoma |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.157G>A (p.Glu53Lys) | Missense | <0.1% | Unknown functional impact |
| c.301C>T (p.Arg101Trp) | Missense | <0.1% | Reported in COSMIC |
| c.424_425insA | Frameshift | <0.1% | Predicted loss of function |
Mutation functional classification
Loss of Function (LOF)
Frameshift and nonsense variants likely lead to loss of function, but rare in tumors.
Gain of Function (GOF)
No well-characterized gain-of-function mutations reported.
Dominant Negative (DN)
Not established for PCLAF.
View complete mutation data：
Gene Ontology (GO)
| • DNA replication | • DNA repair |
| • cell cycle | • chromatin binding |
| • PCNA binding | • nucleus |
Pathways
• PCNA-dependent DNA replication
• Translesion synthesis
• Base excision repair
Protein Summary
PCLAF is a 15 kDa nuclear protein that directly interacts with PCNA via a conserved PIP-box motif. It modulates PCNA loading onto DNA, influences replication fork progression, and participates in DNA damage bypass. Its expression is cell-cycle regulated, peaking in S phase. In cancer, PCLAF overexpression drives proliferation and therapy resistance.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| PCLAF Knockout HEK293 Cell Line | EDJ-KQ6738 | Human | 9768 | Details Get a Quote |
| PCLAF Knockout A-549 Cell Line | EDJ-KQ31135 | Human | 9768 | Details Get a Quote |
| PCLAF Knockout HCT 116 Cell Line | EDJ-KQ31136 | Human | 9768 | Details Get a Quote |
| PCLAF Knockout HeLa Cell Line | EDJ-KQ31137 | Human | 9768 | Details Get a Quote |
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