PARP3: Poly(ADP-Ribose) Polymerase 3
A DNA repair enzyme involved in ADP-ribosylation and genome stability
Gene Information Card
| Symbol | PARP3 |
|---|---|
| Full Name | Poly(ADP-Ribose) Polymerase 3 |
| Gene Type | Protein coding |
| Chromosomal Location | 3p21.2 |
| NCBI Gene ID | 10039 ncbi.nlm.nih.gov/gene/10039 |
| Ensembl ID | ENSG00000163874 |
| UniProt ID | Q9Y6F1 |
| OMIM ID | 607726 |
| HGNC ID | 272 |
| Aliases | ADPRT3, ARTD3, PADPRT-3 |
Description
PARP3 (poly(ADP-ribose) polymerase 3) is a member of the PARP family of enzymes that catalyze the transfer of ADP-ribose from NAD+ to target proteins. It plays a key role in DNA repair, particularly in the response to double-strand breaks, and contributes to genome stability. PARP3 is involved in the regulation of chromatin structure and cell cycle progression.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast cancer | PARP3 overexpression may promote DNA repair in tumor cells, conferring resistance to therapy | ClinVar, COSMIC |
| Ovarian cancer | Altered PARP3 expression linked to DNA repair pathway dysregulation | COSMIC |
| Lung cancer | Mutations and copy number alterations observed in non-small cell lung cancer | COSMIC |
| Colorectal cancer | PARP3 variants associated with tumor progression | COSMIC |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.5 | Medium |
| Spleen | 8.3 | Low |
| Lung | 6.1 | Low |
| Breast | 4.7 | Low |
| Ovary | 3.9 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 10.2 | Cervical cancer cell line |
| A549 | 7.8 | Lung cancer cell line |
| MCF7 | 5.4 | Breast cancer cell line |
| HEK293 | 4.1 | Embryonic kidney cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1234C>T (p.Arg412Cys) | Missense | 0.02% | Unknown functional impact |
| c.567delA (p.Lys189fs) | Frameshift | 0.01% | Loss of function |
| c.890G>A (p.Arg297Gln) | Missense | 0.03% | Reduced catalytic activity |
Mutation functional classification
Loss of Function (LOF)
Frameshift and nonsense mutations that truncate the protein or disrupt the catalytic domain lead to loss of ADP-ribosyltransferase activity.
Gain of Function (GOF)
No well-characterized gain-of-function mutations reported in PARP3.
Dominant Negative (DN)
Some missense mutations in the catalytic domain may interfere with wild-type PARP3 function, but evidence is limited.
View complete mutation data：
Gene Ontology (GO)
| • NAD+ ADP-ribosyltransferase activity (GO:0003950) | • double-strand break repair via nonhomologous end joining (GO:0006303) |
| • nucleus (GO:0005634) | • protein ADP-ribosylation (GO:0006471) |
| • cellular response to DNA damage stimulus (GO:0006974) |
Pathways
• DNA double-strand break repair (non-homologous end joining)
• ADP-ribosylation of proteins
• Base excision repair (indirect)
Protein Summary
PARP3 is a 533-amino acid protein with a molecular weight of approximately 60 kDa. It contains a N-terminal WGR domain and a C-terminal catalytic PARP domain. PARP3 catalyzes mono-ADP-ribosylation of target proteins, including histones and DNA repair factors, and is involved in the early steps of double-strand break repair. It localizes to the nucleus and interacts with other DNA repair proteins such as Ku70/80 and DNA-PKcs.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| PARP3 Knockout HEK293 Cell Line | EDJ-KQ3831 | Human | 10039 | Details Get a Quote |
| PARP3 Knockout A-549 Cell Line | EDJ-KQ25981 | Human | 10039 | Details Get a Quote |
| PARP3 Knockout HCT 116 Cell Line | EDJ-KQ25982 | Human | 10039 | Details Get a Quote |
| PARP3 Knockout HeLa Cell Line | EDJ-KQ25983 | Human | 10039 | Details Get a Quote |
