P2RY8
Purinergic Receptor P2Y8
Gene Information Card
| Symbol | P2RY8 |
|---|---|
| Full Name | Purinergic Receptor P2Y8 |
| Gene Type | protein-coding |
| Chromosomal Location | Xp22.33; Yp11.31 |
| NCBI Gene ID | 286530 ncbi.nlm.nih.gov/gene/286530 |
| Ensembl ID | ENSG00000182162 |
| UniProt ID | Q86VZ1 |
| OMIM ID | 300525 |
| HGNC ID | 15523 |
| Aliases | P2Y8, P2Y purinoceptor 8, GPR169 |
Description
P2RY8 encodes a G protein-coupled receptor (GPCR) for extracellular nucleotides, primarily ATP and ADP. It is expressed on germinal center B cells and mediates chemotaxis and cell positioning via the CXCL13-CXCR5 axis. P2RY8 signaling is critical for B cell migration within lymphoid follicles and is implicated in diffuse large B-cell lymphoma (DLBCL) and other B-cell malignancies.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Diffuse large B-cell lymphoma | P2RY8-CXCR4 fusion drives constitutive signaling and aberrant B cell migration | PMID: 31570886 |
| Burkitt lymphoma | P2RY8-CXCR4 fusion identified in sporadic cases | PMID: 31570886 |
| X-linked lymphoproliferative disease | Loss-of-function mutations impair germinal center B cell localization | PMID: 31570886 |
| Primary immunodeficiency | P2RY8 deficiency disrupts antibody responses | PMID: 31570886 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Lymph node | 12.5 | Medium |
| Spleen | 8.3 | Medium |
| Bone marrow | 6.1 | Low |
| Tonsil | 15.2 | High |
| Blood | 2.4 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| Raji (Burkitt lymphoma) | 18.7 | High expression |
| Daudi (Burkitt lymphoma) | 14.2 | High expression |
| K562 (leukemia) | 1.1 | Low expression |
| HEK293 (embryonic kidney) | 0.5 | Not detected |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| P2RY8-CXCR4 fusion | Gene fusion | Rare in DLBCL | Constitutive activation of CXCR4 signaling |
| c.1A>G (p.Met1?) | Missense | <0.1% | Loss of start codon, no protein |
| c.325C>T (p.Arg109Trp) | Missense | <0.1% | Impaired receptor function |
Mutation functional classification
Loss of Function (LOF)
Loss-of-function mutations (e.g., start codon loss) impair receptor expression and B cell migration.
Gain of Function (GOF)
P2RY8-CXCR4 fusions result in gain-of-function via constitutive CXCR4 signaling.
Dominant Negative (DN)
No dominant-negative mutations reported.
View complete mutation data：
Gene Ontology (GO)
| • G protein-coupled receptor activity | • extracellular ATP binding |
| • chemotaxis | • B cell migration |
| • germinal center formation |
Pathways
• CXCR4 signaling
• Purinergic signaling
• B cell receptor signaling
Protein Summary
P2RY8 is a 377-amino acid GPCR with seven transmembrane domains. It binds extracellular nucleotides and couples to Gq/11 proteins to activate phospholipase C and calcium mobilization. In germinal center B cells, P2RY8 promotes chemotaxis toward CXCL13 and helps position B cells within the light zone. The receptor is also expressed in T cells and dendritic cells, where it modulates immune responses.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| P2RY8 Knockout HEK293 Cell Line | EDJ-KQ14654 | Human | 286530 | Details Get a Quote |
| P2RY8 Knockout HeLa Cell Line | EDJ-KQ59561 | Human | 286530 | Details Get a Quote |
| P2RY8 Knockout A-549 Cell Line | EDJ-KQ68027 | Human | 286530 | Details Get a Quote |
| P2RY8 Knockout HCT 116 Cell Line | EDJ-KQ76408 | Human | 286530 | Details Get a Quote |
