OLA1 (Obg-like ATPase 1)
A conserved ATPase involved in translational regulation and cellular stress response
Gene Information Card
| Symbol | OLA1 |
|---|---|
| Full Name | Obg-like ATPase 1 |
| Gene Type | Protein-coding |
| Chromosomal Location | 2q31.1 |
| NCBI Gene ID | 29789 ncbi.nlm.nih.gov/gene/29789 |
| Ensembl ID | ENSG00000115977 |
| UniProt ID | Q9NTK5 |
| OMIM ID | 611175 |
| HGNC ID | 24983 |
| Aliases | DOC45, GBP45, GTBP45, PTD004 |
Description
OLA1 (Obg-like ATPase 1) encodes a member of the Obg family of P-loop GTPases/ATPases. The protein localizes to the cytoplasm and nucleus, where it functions as a translational regulator and participates in cellular responses to stress, including oxidative stress and DNA damage. OLA1 modulates the activity of the eukaryotic translation initiation factor 2 (eIF2) and interacts with heat shock proteins. It is ubiquitously expressed with highest levels in testis, heart, and skeletal muscle.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast cancer | OLA1 overexpression promotes cell proliferation and invasion; knockdown reduces tumor growth in xenograft models | PMID: 25605248 |
| Colorectal cancer | Upregulation correlates with poor prognosis; OLA1 enhances Wnt/β-catenin signaling | PMID: 27794026 |
| Lung adenocarcinoma | High expression associated with metastasis and shorter survival; OLA1 regulates EMT | PMID: 29367603 |
| Hepatocellular carcinoma | OLA1 silencing suppresses proliferation and induces apoptosis via p53 pathway | PMID: 30351378 |
| Neurodegenerative disorders | OLA1 interacts with tau and modulates tau aggregation in cellular models | PMID: 31577916 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 28.5 | High |
| Heart | 22.1 | High |
| Skeletal muscle | 18.7 | High |
| Brain | 8.3 | Medium |
| Liver | 6.1 | Medium |
| Lung | 5.4 | Medium |
| Kidney | 4.8 | Low |
| Pancreas | 3.2 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 12.3 | Embryonic kidney; high endogenous expression |
| HeLa | 9.8 | Cervical carcinoma; moderate expression |
| MCF7 | 7.5 | Breast cancer; moderate expression |
| A549 | 6.2 | Lung adenocarcinoma; moderate expression |
| HepG2 | 5.1 | Hepatocellular carcinoma; low-moderate expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.101C>T (p.Thr34Met) | Missense | <0.01% | Unknown; rare variant in population databases |
| c.452A>G (p.Asn151Ser) | Missense | <0.01% | Reported in COSMIC; functional impact not characterized |
| c.784G>A (p.Gly262Arg) | Missense | <0.01% | Observed in cancer samples; potential loss-of-function |
| c.1123_1124insA (p.Thr375Asnfs*2) | Frameshift | <0.01% | Predicted loss-of-function; truncating mutation |
Mutation functional classification
Loss of Function (LOF)
Frameshift and nonsense mutations (e.g., p.Thr375Asnfs*2) are predicted to cause loss of ATPase activity and protein truncation.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported; overexpression in tumors suggests potential oncogenic role via increased wild-type activity.
Dominant Negative (DN)
Not described for OLA1.
View complete mutation data：
Gene Ontology (GO)
Pathways
• eIF2 signaling (Reactome R-HSA-156827)
• Unfolded protein response (UPR)
• p53 pathway (KEGG hsa04115)
• Wnt/β-catenin signaling (KEGG hsa04310)
Protein Summary
OLA1 is a 396-amino acid protein (molecular weight ~45 kDa) belonging to the Obg family of P-loop NTPases. It contains a conserved N-terminal GTP-binding domain and a C-terminal domain with ATPase activity. The protein shuttles between cytoplasm and nucleus, and its ATPase activity is essential for regulating translation initiation by modulating eIF2α phosphorylation. OLA1 also interacts with Hsp70/Hsp90 chaperones and is involved in maintaining proteostasis under stress conditions. Overexpression in multiple cancers suggests a role in tumor progression.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| OLA1 Knockout HCT 116 Cell Line | EDJ-KQ17958 | Human | 29789 | Details Get a Quote |
| OLA1 Knockout HeLa Cell Line | EDJ-KQ18142 | Human | 29789 | Details Get a Quote |
| OLA1 Knockout LoVo Cell Line | EDJ-KZ379 | Human | 29789 | Details Get a Quote |
| OLA1 Knockout HEK293 Cell Line | EDJ-KQ51240 | Human | 29789 | Details Get a Quote |
| OLA1 Knockout A-549 Cell Line | EDJ-KQ64595 | Human | 29789 | Details Get a Quote |
