NPFFR2
Neuropeptide FF Receptor 2
Gene Information Card
| Symbol | NPFFR2 |
|---|---|
| Full Name | Neuropeptide FF Receptor 2 |
| Gene Type | protein-coding |
| Chromosomal Location | 4q21.1 |
| NCBI Gene ID | 10886 ncbi.nlm.nih.gov/gene/10886 |
| Ensembl ID | ENSG00000138698 |
| UniProt ID | Q9Y5X5 |
| OMIM ID | 607620 |
| HGNC ID | 4526 |
| Aliases | GPR74, NPGPR, HLWAR77 |
Description
NPFFR2 (neuropeptide FF receptor 2) is a G protein-coupled receptor that binds neuropeptide FF (NPFF) and related peptides. It is involved in pain modulation, opioid analgesia modulation, and regulation of food intake and cardiovascular function. The receptor is expressed in the central nervous system and peripheral tissues.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Pain disorders | NPFFR2 modulates nociception via Gi/o signaling; altered expression may affect pain sensitivity | PMID: 12023984 |
| Opioid tolerance | NPFFR2 activation counteracts opioid-induced analgesia; chronic opioid use may upregulate NPFFR2 | PMID: 15987666 |
| Hypertension | NPFFR2 expressed in cardiovascular centers; NPFF binding influences blood pressure regulation | PMID: 16919457 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 12.5 | Medium |
| Spinal cord | 8.3 | Low |
| Heart | 4.1 | Low |
| Kidney | 3.2 | Low |
| Testis | 2.8 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| SH-SY5Y | 5.6 | Neuroblastoma cell line; moderate expression |
| HEK293 | 2.1 | Low expression; used for heterologous expression studies |
| U-87 MG | 1.8 | Glioblastoma cell line; low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.100C>T (p.Arg34Cys) | Missense | <0.01% | Unknown functional effect; rare variant in population databases |
| c.457G>A (p.Val153Met) | Missense | <0.01% | Predicted benign; no disease association reported |
Mutation functional classification
Loss of Function (LOF)
No confirmed loss-of-function mutations reported in NPFFR2.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported in NPFFR2.
Dominant Negative (DN)
No dominant-negative mutations reported in NPFFR2.
View complete mutation data：
Gene Ontology (GO)
| • G protein-coupled receptor activity | • neuropeptide FF receptor activity |
| • signal transduction | • G protein-coupled receptor signaling pathway |
| • modulation of chemical synaptic transmission | • response to pain |
Pathways
• Neuropeptide FF receptor signaling
• GPCR downstream signaling
• Opioid signaling pathway (modulatory)
Protein Summary
NPFFR2 is a 7-transmembrane G protein-coupled receptor of 522 amino acids. It couples primarily to Gi/o proteins, inhibiting adenylyl cyclase and modulating ion channels. The receptor is activated by neuropeptide FF (NPFF) and related RFamide peptides, playing roles in pain modulation, opioid tolerance, and cardiovascular regulation.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| NPFFR2 Knockout HEK293 Cell Line | EDJ-KQ7202 | Human | 10886 | Details Get a Quote |
| NPFFR2 Knockout HeLa Cell Line | EDJ-KQ55515 | Human | 10886 | Details Get a Quote |
| NPFFR2 Knockout A-549 Cell Line | EDJ-KQ64005 | Human | 10886 | Details Get a Quote |
| NPFFR2 Knockout HCT 116 Cell Line | EDJ-KQ72456 | Human | 10886 | Details Get a Quote |
