NPFFR1
Neuropeptide FF Receptor 1
Gene Information Card
| Symbol | NPFFR1 |
|---|---|
| Full Name | Neuropeptide FF Receptor 1 |
| Gene Type | protein-coding |
| Chromosomal Location | 10q21.3 |
| NCBI Gene ID | 64106 ncbi.nlm.nih.gov/gene/64106 |
| Ensembl ID | ENSG00000148734 |
| UniProt ID | Q9GZQ4 |
| OMIM ID | 607646 |
| HGNC ID | 17320 |
| Aliases | GPR147, NPFF1, OT7T022 |
Description
NPFFR1 encodes a G protein-coupled receptor for neuropeptide FF (NPFF) and related peptides. It is involved in pain modulation, opioid tolerance, and neuroendocrine signaling. The receptor is expressed in the central nervous system and peripheral tissues.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Pain disorders | Receptor activation modulates nociception via Gi/o signaling | PMID: 12023984 |
| Opioid tolerance | NPFFR1 antagonism reduces tolerance development | PMID: 15689462 |
| Hypertension | NPFFR1 expression in cardiovascular centers affects blood pressure | PMID: 17015439 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 12.5 | Medium |
| Spinal cord | 8.3 | Low |
| Testis | 6.1 | Low |
| Kidney | 4.2 | Low |
| Heart | 3.8 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| SH-SY5Y | 5.2 | Neuroblastoma cell line |
| HEK293 | 2.1 | Low endogenous expression |
| U-87 MG | 1.5 | Glioblastoma cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.200G>A (p.Arg67His) | Missense | <0.01% | Reduced ligand binding affinity (ClinVar) |
| c.487C>T (p.Arg163Cys) | Missense | <0.01% | Altered receptor internalization (ClinVar) |
Mutation functional classification
Loss of Function (LOF)
p.Arg67His reduces NPFF binding and downstream signaling.
Gain of Function (GOF)
No confirmed gain-of-function variants reported.
Dominant Negative (DN)
No evidence for dominant-negative effects.
View complete mutation data：
Gene Ontology (GO)
| • G protein-coupled receptor activity | • neuropeptide Y receptor activity |
| • plasma membrane | • signal transduction |
| • adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway |
Pathways
• Neuropeptide FF receptor signaling
• Gi alpha signaling events
• Opioid signaling
Protein Summary
NPFFR1 is a 7-transmembrane G protein-coupled receptor that binds neuropeptide FF and related RFamide peptides. It couples primarily to Gi/o proteins, inhibiting cAMP production. The receptor modulates pain perception, opioid analgesia, and cardiovascular function.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| NPFFR1 Knockout HEK293 Cell Line | EDJ-KQ14461 | Human | 64106 | Details Get a Quote |
| NPFFR1 Knockout HeLa Cell Line | EDJ-KQ57028 | Human | 64106 | Details Get a Quote |
| NPFFR1 Knockout A-549 Cell Line | EDJ-KQ65534 | Human | 64106 | Details Get a Quote |
| NPFFR1 Knockout HCT 116 Cell Line | EDJ-KQ73969 | Human | 64106 | Details Get a Quote |
