NEK6: NIMA Related Kinase 6
A serine/threonine kinase involved in cell cycle regulation and cancer
Gene Information Card
| Symbol | NEK6 |
|---|---|
| Full Name | NIMA related kinase 6 |
| Gene Type | protein-coding |
| Chromosomal Location | 9q33.3 |
| NCBI Gene ID | 10783 ncbi.nlm.nih.gov/gene/10783 |
| Ensembl ID | ENSG00000120833 |
| UniProt ID | Q9HC98 |
| OMIM ID | 604884 |
| HGNC ID | 7748 |
| Aliases | SID6-1512, FLJ21959, MGC44427 |
Description
NEK6 (NIMA related kinase 6) is a serine/threonine protein kinase that plays a critical role in cell cycle progression, particularly in mitotic spindle formation and cytokinesis. It is a member of the NEK family of kinases, which are involved in various cellular processes including DNA damage response and apoptosis. NEK6 is frequently overexpressed or mutated in multiple cancer types, making it a potential therapeutic target.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast cancer | Overexpression and amplification of NEK6 promote cell proliferation and resistance to apoptosis | PMID: 23431136; COSMIC |
| Colorectal cancer | NEK6 upregulation correlates with poor prognosis and metastasis | PMID: 25605247 |
| Prostate cancer | NEK6 knockdown reduces tumor growth in xenograft models | PMID: 27323851 |
| Lung cancer | NEK6 mutations and overexpression linked to oncogenic signaling | PMID: 28411373; COSMIC |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.5 | Medium |
| Bone marrow | 8.3 | Medium |
| Lymph node | 6.1 | Low |
| Brain | 2.4 | Low |
| Liver | 1.8 | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 15.2 | Cervical cancer cell line |
| MCF7 | 11.8 | Breast cancer cell line |
| A549 | 9.5 | Lung cancer cell line |
| HEK293 | 7.3 | Embryonic kidney cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.104C>T (p.Thr35Met) | Missense | 0.2% | Unknown functional effect |
| c.487G>A (p.Glu163Lys) | Missense | 0.1% | Potential loss of kinase activity |
| c.832A>G (p.Asn278Asp) | Missense | 0.05% | Reported in COSMIC; uncertain significance |
Mutation functional classification
Loss of Function (LOF)
p.Glu163Lys may reduce kinase activity based on structural modeling
Gain of Function (GOF)
Not well characterized; some mutations may increase activity in cancer
Dominant Negative (DN)
No confirmed dominant-negative mutations reported
View complete mutation data：
Gene Ontology (GO)
Pathways
• Cell Cycle
• Mitotic (Reactome: R-HSA-69278)
• Signaling by NGF (Reactome: R-HSA-166520)
• p53 signaling pathway (KEGG: hsa04115)
Protein Summary
NEK6 is a 313-amino acid serine/threonine kinase with an N-terminal catalytic domain. It localizes to the centrosome and spindle poles during mitosis. NEK6 phosphorylates substrates such as kinesin-like protein KIF11 and histone H3, regulating chromosome segregation. Its activity is controlled by autophosphorylation and interaction with NEK9. Overexpression in tumors suggests a role in oncogenesis.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| NEK6 Knockout HEK293 Cell Line | EDJ-KQ2822 | Human | 10783 | Details Get a Quote |
| NEK6 Knockout A-549 Cell Line | EDJ-KQ23798 | Human | 10783 | Details Get a Quote |
| NEK6 Knockout HCT 116 Cell Line | EDJ-KQ23799 | Human | 10783 | Details Get a Quote |
| NEK6 Knockout HeLa Cell Line | EDJ-KQ23800 | Human | 10783 | Details Get a Quote |
