NADK: NAD Kinase
Key enzyme in NADP biosynthesis, linking metabolism to cellular redox and signaling
Gene Information Card
| Symbol | NADK |
|---|---|
| Full Name | NAD Kinase |
| Gene Type | protein-coding |
| Chromosomal Location | 1p36.33 |
| NCBI Gene ID | 65220 ncbi.nlm.nih.gov/gene/65220 |
| Ensembl ID | ENSG00000142611 |
| UniProt ID | O95544 |
| OMIM ID | 611636 |
| HGNC ID | 29831 |
| Aliases | NADK1, dJ283E3.1 |
Description
NADK encodes NAD kinase, the enzyme that catalyzes the phosphorylation of NAD+ to NADP+ using ATP as a phosphate donor. This reaction is essential for maintaining the cellular NADP+/NADPH pool, which is critical for reductive biosynthesis, antioxidant defense, and signaling. NADK is ubiquitously expressed and its activity is regulated by calcium/calmodulin and cellular energy status.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Cancer (various) | Altered NADK expression affects NADPH levels, influencing redox balance and anabolic pathways in tumor cells. | PMID: 25609688; COSMIC overexpression in multiple cancer types |
| Metabolic syndrome | NADK deficiency impairs NADPH production, linked to oxidative stress and insulin resistance. | PMID: 23395176 |
| Neurodegeneration | Reduced NADK activity compromises neuronal antioxidant capacity, implicated in models of Parkinson's and Alzheimer's disease. | PMID: 27511111 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Liver | 12.5 | Medium |
| Kidney | 10.2 | Medium |
| Heart | 8.9 | Medium |
| Brain | 6.3 | Low |
| Skeletal Muscle | 5.1 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 14.0 | High expression |
| HeLa | 11.5 | Medium expression |
| HepG2 | 10.8 | Medium expression |
| K562 | 7.2 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1A>G (p.Met1?) | missense | <0.01% | Likely loss of start codon; predicted loss of function |
| c.487C>T (p.Arg163Trp) | missense | <0.01% | Reduced catalytic activity in vitro |
| c.1042G>A (p.Glu348Lys) | missense | <0.01% | Unknown functional effect |
Mutation functional classification
Loss of Function (LOF)
Mutations affecting the ATP-binding or catalytic domain (e.g., p.Arg163Trp) reduce NAD kinase activity, impairing NADP+ synthesis.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported in NADK.
Dominant Negative (DN)
No evidence of dominant-negative effects for NADK mutations.
View complete mutation data：
Gene Ontology (GO)
| • NAD+ kinase activity (GO:0003951) | • ATP binding (GO:0005524) |
| • NADP biosynthetic process (GO:0006741) | • cytoplasm (GO:0005737) |
| • cytosol (GO:0005829) |
Pathways
• NAD+ kinase pathway (Reactome: R-HSA-196807)
• Nicotinate and nicotinamide metabolism (KEGG: hsa00760)
• Metabolic pathways (KEGG: hsa01100)
Protein Summary
NADK is a 49 kDa cytosolic enzyme that phosphorylates NAD+ to NADP+ using ATP. It contains a conserved NAD kinase domain and is regulated by calcium/calmodulin binding. The enzyme is essential for maintaining the NADP+/NADPH pool, supporting reductive biosynthesis and antioxidant defense. NADK is ubiquitously expressed with highest levels in liver and kidney.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| NADK2 Knockout HEK293 Cell Line | EDJ-KQ9321 | Human | 133686 | Details Get a Quote |
| NADK Knockout HEK293 Cell Line | EDJ-KQ14363 | Human | 65220 | Details Get a Quote |
| NADK2 Knockout A-549 Cell Line | EDJ-KQ35946 | Human | 133686 | Details Get a Quote |
| NADK2 Knockout HeLa Cell Line | EDJ-KQ35948 | Human | 133686 | Details Get a Quote |
| NADK Knockout A-549 Cell Line | EDJ-KQ44494 | Human | 65220 | Details Get a Quote |
| NADK Knockout HCT 116 Cell Line | EDJ-KQ44495 | Human | 65220 | Details Get a Quote |
| NADK Knockout HeLa Cell Line | EDJ-KQ44496 | Human | 65220 | Details Get a Quote |
| NADK2 Knockout HCT 116 Cell Line | EDJ-KQ34703 | Human | 133686 | Details Get a Quote |
Displaying Records 1 To 8 Of 8 Records
