MYLK4: Myosin Light Chain Kinase Family Member 4
A pseudokinase with potential roles in cell signaling and cancer
Gene Information Card
| Symbol | MYLK4 |
|---|---|
| Full Name | myosin light chain kinase family member 4 |
| Gene Type | protein-coding (pseudokinase) |
| Chromosomal Location | 6p22.3 |
| NCBI Gene ID | 340156 ncbi.nlm.nih.gov/gene/340156 |
| Ensembl ID | ENSG00000188827 |
| UniProt ID | Q86YV6 |
| OMIM ID | 615399 |
| HGNC ID | 27972 |
| Aliases | MLCK4, MLCK-4, caMLCK |
Description
MYLK4 encodes a member of the myosin light chain kinase (MLCK) family. The protein contains a kinase domain but lacks critical catalytic residues, classifying it as a pseudokinase. It may regulate cytoskeletal dynamics and cell migration through non-catalytic interactions. Expression is enriched in testis and certain cancer cell lines.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Cancer (general) | Altered expression may affect cell motility and invasion; mutations observed in various tumors | COSMIC |
| Breast cancer | Overexpression in some subtypes; potential role in metastasis | COSMIC, literature |
| Colorectal cancer | Somatic mutations reported; functional significance unclear | COSMIC |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.5 | Medium |
| Lymph node | 6.8 | Low |
| Spleen | 5.2 | Low |
| Bone marrow | 4.1 | Low |
| Brain | 2.3 | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 8.9 | Moderate expression |
| HeLa | 6.5 | Low expression |
| MCF7 | 7.2 | Low expression |
| A549 | 5.0 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| p.R89W | Missense | <0.1% | Unknown; possibly damaging |
| p.G120E | Missense | <0.1% | Unknown |
| p.S456L | Missense | <0.1% | Unknown |
Mutation functional classification
Loss of Function (LOF)
Not established; pseudokinase activity may be dispensable
Gain of Function (GOF)
Not reported
Dominant Negative (DN)
Not reported
View complete mutation data：
Gene Ontology (GO)
| • protein kinase activity (inferred) | • ATP binding (inferred) |
| • cytoskeleton organization | • cell migration |
Pathways
• Myosin light chain kinase pathway (inferred)
• Regulation of actin cytoskeleton
Protein Summary
MYLK4 is a 561-amino acid pseudokinase with a degenerate catalytic domain. It localizes to the cytoplasm and may interact with actin filaments. Its precise molecular function remains unclear, but it is thought to modulate cell shape and motility.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| MYLK4 Knockout HEK293 Cell Line | EDJ-KQ1437 | Human | 340156 | Details Get a Quote |
| MYLK4 Knockout HeLa Cell Line | EDJ-KQ59668 | Human | 340156 | Details Get a Quote |
| MYLK4 Knockout A-549 Cell Line | EDJ-KQ68140 | Human | 340156 | Details Get a Quote |
| MYLK4 Knockout HCT 116 Cell Line | EDJ-KQ76517 | Human | 340156 | Details Get a Quote |
