MPG (N-Methylpurine DNA Glycosylase) Gene
Key player in base excision repair pathway
Gene Information Card
| Symbol | MPG |
|---|---|
| Full Name | N-methylpurine DNA glycosylase |
| Gene Type | Protein coding |
| Chromosomal Location | 16p13.3 |
| NCBI Gene ID | 4350 ncbi.nlm.nih.gov/gene/4350 |
| Ensembl ID | ENSG00000103152 |
| UniProt ID | P29372 |
| OMIM ID | 131675 |
| HGNC ID | 7214 |
| Aliases | AAG, ANPG, APNG, MID1, PIG11 |
Description
The MPG gene encodes N-methylpurine DNA glycosylase, a DNA repair enzyme that initiates base excision repair by removing damaged bases such as 3-methyladenine, 7-methylguanine, and hypoxanthine from DNA. It is involved in the cellular response to alkylating agents and oxidative stress.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Colorectal Cancer | Altered MPG expression may affect repair of alkylation damage, contributing to genomic instability | PMID: 23431129 |
| Lung Cancer | Polymorphisms in MPG associated with increased risk | PMID: 20068074 |
| Breast Cancer | MPG overexpression linked to resistance to alkylating chemotherapy | PMID: 16951153 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.5 | Medium |
| Liver | 8.3 | Medium |
| Kidney | 7.1 | Medium |
| Brain | 4.2 | Low |
| Heart | 3.8 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 10.2 | Cervical cancer cell line |
| A549 | 9.5 | Lung cancer cell line |
| HEK293 | 8.9 | Embryonic kidney cell line |
| MCF7 | 7.6 | Breast cancer cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.145C>T (p.Arg49Cys) | Missense | Rare | Reduced glycosylase activity |
| c.293A>G (p.Asn98Ser) | Missense | Rare | Altered substrate specificity |
| c.415G>A (p.Gly139Arg) | Missense | Rare | Unknown functional effect |
Mutation functional classification
Loss of Function (LOF)
Missense mutations such as p.Arg49Cys reduce enzymatic activity, impairing base excision repair.
Gain of Function (GOF)
No gain-of-function mutations reported.
Dominant Negative (DN)
No dominant-negative mutations reported.
View complete mutation data：
Gene Ontology (GO)
| • base-excision repair (GO:0006284) | • DNA-(apurinic or apyrimidinic site) lyase activity (GO:0003906) |
| • DNA-3-methyladenine glycosylase activity (GO:0008725) | • nucleus (GO:0005634) |
Pathways
• Base excision repair (BER) – Reactome R-HSA-73929
• DNA repair – KEGG hsa03410
Protein Summary
The MPG protein is a monomeric DNA glycosylase that recognizes and removes a variety of alkylated and deaminated purine bases. It has a conserved helix-hairpin-helix (HhH) motif and a catalytic aspartic acid residue. The enzyme is localized to the nucleus and is expressed ubiquitously.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| MPG Knockout HEK293 Cell Line | EDJ-KQ5236 | Human | 4350 | Details Get a Quote |
| MPG Knockout HeLa Cell Line | EDJ-KQ27027 | Human | 4350 | Details Get a Quote |
| MPG Knockout A-549 Cell Line | EDJ-KQ28262 | Human | 4350 | Details Get a Quote |
| MPG Knockout HCT 116 Cell Line | EDJ-KQ28263 | Human | 4350 | Details Get a Quote |
