MED28 Gene - Mediator Complex Subunit 28
A component of the Mediator complex involved in transcriptional regulation and linked to cancer and developmental disorders.
Gene Information Card
| Symbol | MED28 |
|---|---|
| Full Name | Mediator Complex Subunit 28 |
| Gene Type | Protein coding |
| Chromosomal Location | 4p15.32 |
| NCBI Gene ID | 80306 ncbi.nlm.nih.gov/gene/80306 |
| Ensembl ID | ENSG00000138668 |
| UniProt ID | Q9H204 |
| OMIM ID | 610311 |
| HGNC ID | 24636 |
| Aliases | EG1, magicin, 1500003D12Rik |
Description
MED28 encodes a subunit of the Mediator complex, a multiprotein coactivator required for RNA polymerase II transcription. The protein interacts with other Mediator subunits and transcription factors, playing roles in cell proliferation, differentiation, and cytoskeletal organization. MED28 is also known as magicin and has been implicated in cancer and developmental processes.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast cancer | Overexpression of MED28 promotes cell proliferation and migration; potential oncogenic role | PMID: 21573169 |
| Colorectal cancer | Upregulation associated with poor prognosis; may enhance Wnt signaling | PMID: 25132229 |
| Gastric cancer | Increased expression linked to tumor progression and metastasis | PMID: 27323883 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 15.2 | Medium |
| Thyroid | 12.8 | Medium |
| Prostate | 10.5 | Medium |
| Breast | 8.3 | Low |
| Colon | 7.1 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| MCF7 (breast cancer) | 12.0 | Moderate expression |
| HCT116 (colorectal cancer) | 14.5 | Moderate-high expression |
| HEK293 (embryonic kidney) | 9.8 | Low-moderate expression |
| K562 (leukemia) | 6.2 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.287A>G (p.Asn96Ser) | Missense | <0.01% | Unknown; rare variant in population databases |
| c.454C>T (p.Arg152Trp) | Missense | <0.01% | Reported in COSMIC; potential loss of function |
| c.631_633del (p.Lys211del) | In-frame deletion | <0.01% | Unknown functional effect |
Mutation functional classification
Loss of Function (LOF)
No confirmed loss-of-function mutations reported in ClinVar or COSMIC.
Gain of Function (GOF)
Overexpression in several cancers suggests potential gain-of-function role, but no specific activating mutations identified.
Dominant Negative (DN)
No evidence for dominant-negative mutations.
View complete mutation data：
Gene Ontology (GO)
| • transcription coactivator activity (GO:0003712) | • nucleus (GO:0005634) |
| • mediator complex (GO:0016592) | • regulation of transcription by RNA polymerase II (GO:0006357) |
| • cell cycle (GO:0007049) |
Pathways
• Mediator complex (Reactome: R-HSA-212436)
• Transcriptional regulation by TP53 (Reactome: R-HSA-3700989)
• Wnt signaling pathway (KEGG: hsa04310)
Protein Summary
MED28 is a 178-amino acid protein (20 kDa) that localizes to the nucleus and cytoplasm. It contains a coiled-coil domain and interacts with other Mediator subunits (e.g., MED1, MED24) and cytoskeletal proteins. The protein is involved in transcriptional coactivation and may regulate cell adhesion and migration. Its expression is elevated in several cancers, suggesting a role in tumorigenesis.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| MED28 Knockout HEK293 Cell Line | EDJ-KQ51745 | Human | 80306 | Details Get a Quote |
| MED28 Knockout HeLa Cell Line | EDJ-KQ57324 | Human | 80306 | Details Get a Quote |
| MED28 Knockout A-549 Cell Line | EDJ-KQ65830 | Human | 80306 | Details Get a Quote |
| MED28 Knockout HCT 116 Cell Line | EDJ-KQ74254 | Human | 80306 | Details Get a Quote |
