MED1 Gene: Mediator Complex Subunit 1
A key transcriptional coactivator in gene regulation and cancer
Gene Information Card
| Symbol | MED1 |
|---|---|
| Full Name | Mediator Complex Subunit 1 |
| Gene Type | Protein coding |
| Chromosomal Location | 17q12 |
| NCBI Gene ID | 5469 ncbi.nlm.nih.gov/gene/5469 |
| Ensembl ID | ENSG00000131981 |
| UniProt ID | Q15648 |
| OMIM ID | 604311 |
| HGNC ID | 6918 |
| Aliases | TRAP220, PPARBP, DRIP205, PBP, CRSP1, RB18A |
Description
MED1 encodes a subunit of the Mediator complex, a multiprotein coactivator required for RNA polymerase II transcription. MED1 bridges transcription factors (e.g., nuclear receptors) and the basal transcription machinery, playing a critical role in cell growth, differentiation, and metabolism. It is frequently altered in cancers and metabolic disorders.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast cancer | MED1 amplification/overexpression drives ERα-mediated transcription and tamoxifen resistance | PMID: 15626733; COSMIC |
| Prostate cancer | MED1 interacts with androgen receptor to promote proliferation and therapy resistance | PMID: 20010870; ClinVar |
| Metabolic syndrome | MED1 deficiency impairs PPARγ signaling, leading to insulin resistance and lipodystrophy | PMID: 11867748; OMIM |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Breast | nTPM: 15.2 | Medium |
| Liver | nTPM: 12.8 | Medium |
| Prostate | nTPM: 10.5 | Medium |
| Adipose tissue | nTPM: 8.3 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| MCF7 (breast cancer) | nTPM: 18.5 | High expression; ER+ line |
| LNCaP (prostate cancer) | nTPM: 14.2 | Androgen-responsive |
| HepG2 (liver cancer) | nTPM: 11.0 | Moderate expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.2935C>T (p.Arg979*) | Nonsense | <1% | Loss of function; truncation |
| c.1234A>G (p.Lys412Glu) | Missense | <0.5% | Unknown; predicted damaging |
| Amplification (17q12) | Copy number gain | 5-10% in breast cancer | Increased MED1 expression |
Mutation functional classification
Loss of Function (LOF)
Nonsense and frameshift mutations reduce MED1 protein levels, impairing Mediator complex assembly and transcription of target genes.
Gain of Function (GOF)
Gene amplification leads to MED1 overexpression, enhancing coactivation of oncogenic transcription factors (e.g., ERα, AR).
Dominant Negative (DN)
Truncated MED1 variants may interfere with Mediator complex function, but dominant-negative effects are not well documented.
View complete mutation data：
Gene Ontology (GO)
Pathways
• Mediator complex (Reactome: R-HSA-212436)
• PPAR signaling pathway (KEGG: hsa03320)
• Estrogen signaling pathway (KEGG: hsa04915)
Protein Summary
MED1 (also known as TRAP220, PBP) is a 1581-amino-acid protein that serves as a scaffold within the Mediator complex. It contains nuclear receptor interaction motifs (LXXLL) that bind ligand-activated receptors such as ERα, PPARγ, and AR. MED1 facilitates recruitment of RNA polymerase II and general transcription factors to target gene promoters. Post-translational modifications (e.g., phosphorylation) regulate its activity and stability.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| MED13 Knockout HEK293 Cell Line | EDJ-KQ6846 | Human | 9969 | Details Get a Quote |
| MED16 Knockout HEK293 Cell Line | EDJ-KQ6871 | Human | 10025 | Details Get a Quote |
| TMED1 Knockout HEK293 Cell Line | EDJ-KQ7247 | Human | 11018 | Details Get a Quote |
| MED12L Knockout HEK293 Cell Line | EDJ-KQ7585 | Human | 116931 | Details Get a Quote |
| MED13L Knockout HEK293 Cell Line | EDJ-KQ8001 | Human | 23389 | Details Get a Quote |
| MED10 Knockout HEK293 Cell Line | EDJ-KQ10026 | Human | 84246 | Details Get a Quote |
| MED15 Knockout HEK293 Cell Line | EDJ-KQ11151 | Human | 51586 | Details Get a Quote |
| MED16 Knockout HeLa Cell Line | EDJ-KQ30083 | Human | 10025 | Details Get a Quote |
| MED10 Knockout A-549 Cell Line | EDJ-KQ37019 | Human | 84246 | Details Get a Quote |
| MED10 Knockout HCT 116 Cell Line | EDJ-KQ37020 | Human | 84246 | Details Get a Quote |
| MED10 Knockout HeLa Cell Line | EDJ-KQ37021 | Human | 84246 | Details Get a Quote |
| MED15 Knockout A-549 Cell Line | EDJ-KQ39154 | Human | 51586 | Details Get a Quote |
| MED15 Knockout HCT 116 Cell Line | EDJ-KQ39155 | Human | 51586 | Details Get a Quote |
| MED15 Knockout HeLa Cell Line | EDJ-KQ39156 | Human | 51586 | Details Get a Quote |
| TMED1 Knockout HeLa Cell Line | EDJ-KQ30859 | Human | 11018 | Details Get a Quote |
Displaying Records 1 To 15 Of 30 Records
