MAST3: Microtubule Associated Serine/Threonine Kinase 3
A member of the MAST family of kinases involved in cell polarity, cytoskeletal dynamics, and immune signaling.
Gene Information Card
| Symbol | MAST3 |
|---|---|
| Full Name | Microtubule Associated Serine/Threonine Kinase 3 |
| Gene Type | protein-coding |
| Chromosomal Location | 19p13.11 |
| NCBI Gene ID | 23031 ncbi.nlm.nih.gov/gene/23031 |
| Ensembl ID | ENSG00000105699 |
| UniProt ID | O60307 |
| OMIM ID | 609527 |
| HGNC ID | 18980 |
| Aliases | KIAA0561, MAST3_HUMAN |
Description
MAST3 (Microtubule Associated Serine/Threonine Kinase 3) is a protein-coding gene that encodes a member of the MAST family of serine/threonine kinases. The protein contains a kinase domain and a PDZ domain, and is involved in microtubule dynamics, cell polarity, and immune signaling. MAST3 has been implicated in inflammatory bowel disease and certain cancers.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Inflammatory Bowel Disease (IBD) | MAST3 variants may alter kinase activity affecting NF-κB signaling and immune response. | GWAS studies (PMID: 21102463) |
| Colorectal Cancer | MAST3 overexpression or mutation may promote tumor cell proliferation and migration. | COSMIC database; PMID: 23505284 |
| Breast Cancer | MAST3 copy number alterations and expression changes observed in breast tumors. | TCGA data; COSMIC |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 12.5 | Medium |
| Colon | 8.3 | Medium |
| Breast | 6.1 | Low |
| Lung | 5.4 | Low |
| Liver | 3.2 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 15.2 | High expression |
| HeLa | 9.8 | Medium expression |
| HCT116 | 7.5 | Medium expression |
| MCF7 | 4.3 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1492C>T (p.Arg498Trp) | Missense | <0.01% | Unknown; reported in IBD |
| c.2155G>A (p.Glu719Lys) | Missense | <0.01% | Unknown; reported in colorectal cancer |
| c.1234_1235insA | Frameshift | <0.01% | Loss of function |
Mutation functional classification
Loss of Function (LOF)
Frameshift mutations (e.g., c.1234_1235insA) predicted to truncate the protein and abolish kinase activity.
Gain of Function (GOF)
Not well characterized; some missense variants may enhance kinase activity.
Dominant Negative (DN)
Not reported for MAST3.
View complete mutation data：
Gene Ontology (GO)
Pathways
• MAPK signaling pathway (Reactome R-HSA-5683057)
• Signaling by Receptor Tyrosine Kinases (Reactome R-HSA-9006934)
• NF-kappaB signaling (KEGG hsa04064)
Protein Summary
The MAST3 protein is a 1,070-amino acid serine/threonine kinase that localizes to microtubules and the cytoplasm. It contains an N-terminal kinase domain and a C-terminal PDZ domain. MAST3 is involved in regulating cell polarity, microtubule stability, and immune signaling through phosphorylation of substrates such as PTEN and NF-κB pathway components. Alternative splicing generates multiple isoforms.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| MAST3 Knockout HEK293 Cell Line | EDJ-KQ7102 | Human | 23031 | Details Get a Quote |
| MAST3 Knockout A-549 Cell Line | EDJ-KQ33279 | Human | 23031 | Details Get a Quote |
| MAST3 Knockout HCT 116 Cell Line | EDJ-KQ33280 | Human | 23031 | Details Get a Quote |
| MAST3 Knockout HeLa Cell Line | EDJ-KQ33281 | Human | 23031 | Details Get a Quote |
