MAST2: Microtubule Associated Serine/Threonine Kinase 2
A member of the microtubule-associated serine/threonine kinase family involved in cell polarity, migration, and cancer signaling.
Gene Information Card
| Symbol | MAST2 |
|---|---|
| Full Name | Microtubule Associated Serine/Threonine Kinase 2 |
| Gene Type | protein-coding |
| Chromosomal Location | 1p34.1 |
| NCBI Gene ID | 23139 ncbi.nlm.nih.gov/gene/23139 |
| Ensembl ID | ENSG00000117519 |
| UniProt ID | Q6P0Q8 |
| OMIM ID | 612257 |
| HGNC ID | 19034 |
| Aliases | KIAA0807, MAST205 |
Description
MAST2 (Microtubule Associated Serine/Threonine Kinase 2) encodes a member of the microtubule-associated serine/threonine kinase (MAST) family. The protein contains a serine/threonine kinase domain and a PDZ domain, and is involved in regulating cell polarity, microtubule dynamics, and cell migration. MAST2 has been implicated in cancer progression, particularly through interactions with the tumor suppressor PTEN and the Hippo signaling pathway.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast cancer | MAST2 overexpression promotes cell migration and invasion via PTEN phosphorylation and AKT activation. | PMID: 25605249 |
| Colorectal cancer | MAST2 amplification and overexpression correlate with poor prognosis and activation of Wnt/β-catenin signaling. | PMID: 27562873 |
| Glioblastoma | MAST2 knockdown reduces tumor growth and invasion in vitro and in vivo. | PMID: 29367642 |
| Prostate cancer | MAST2 expression is upregulated and associated with metastasis. | PMID: 30356099 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.5 | Medium |
| Brain | 8.2 | Medium |
| Breast | 6.1 | Low |
| Colon | 5.8 | Low |
| Prostate | 4.9 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| MCF7 (breast cancer) | 10.3 | High expression |
| HCT116 (colorectal cancer) | 9.1 | High expression |
| U87MG (glioblastoma) | 7.8 | Medium expression |
| PC3 (prostate cancer) | 6.5 | Medium expression |
| HEK293 (embryonic kidney) | 4.2 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.2152C>T (p.Arg718Trp) | Missense | <0.1% | Unknown functional effect; reported in COSMIC |
| c.2870A>G (p.Asn957Ser) | Missense | <0.1% | Unknown functional effect; reported in COSMIC |
| c.1123_1124insA (p.Thr375Asnfs*2) | Frameshift | <0.1% | Predicted loss of function |
| c.1745G>A (p.Arg582Gln) | Missense | <0.1% | Unknown functional effect |
Mutation functional classification
Loss of Function (LOF)
Frameshift mutations (e.g., p.Thr375Asnfs*2) are predicted to cause loss of function by truncating the kinase domain.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported in MAST2.
Dominant Negative (DN)
No confirmed dominant-negative mutations reported in MAST2.
View complete mutation data：
Gene Ontology (GO)
| • GO:0004672 (protein kinase activity) | • GO:0004674 (protein serine/threonine kinase activity) |
| • GO:0005515 (protein binding) | • GO:0005737 (cytoplasm) |
| • GO:0005813 (centrosome) | • GO:0005874 (microtubule) |
| • GO:0016310 (phosphorylation) | • GO:0030030 (cell projection organization) |
| • GO:0035556 (intracellular signal transduction) | • GO:0046777 (protein autophosphorylation) |
Pathways
• Hippo signaling pathway (Reactome: R-HSA-2028269)
• Signaling by PTEN (Reactome: R-HSA-6807070)
• MAPK signaling pathway (KEGG: hsa04010)
• Wnt signaling pathway (KEGG: hsa04310)
Protein Summary
MAST2 is a 1,598-amino acid serine/threonine kinase that localizes to microtubules and centrosomes. It contains an N-terminal kinase domain and a C-terminal PDZ domain. MAST2 phosphorylates PTEN, leading to PTEN stabilization and regulation of AKT signaling. It also interacts with the Hippo pathway components MST1/2 and YAP, modulating cell proliferation and migration. Overexpression of MAST2 is observed in multiple cancers and correlates with poor prognosis.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| MAST2 Knockout HEK293 Cell Line | EDJ-KQ2629 | Human | 23139 | Details Get a Quote |
| MAST2 Knockout A-549 Cell Line | EDJ-KQ23370 | Human | 23139 | Details Get a Quote |
| MAST2 Knockout HCT 116 Cell Line | EDJ-KQ23371 | Human | 23139 | Details Get a Quote |
| MAST2 Knockout HeLa Cell Line | EDJ-KQ23372 | Human | 23139 | Details Get a Quote |
| MAST2 Knockout HAP1 Cell Line | EDC08041 | Human | 23139 | Details Get a Quote |
Displaying Records 1 To 5 Of 5 Records
