MAPRE1: Microtubule-Associated Protein RP/EB Family Member 1
A key regulator of microtubule dynamics and cell polarity, implicated in cancer and ciliopathies.
Gene Information Card
| Symbol | MAPRE1 |
|---|---|
| Full Name | Microtubule Associated Protein RP/EB Family Member 1 |
| Gene Type | protein-coding |
| Chromosomal Location | 20q11.21 |
| NCBI Gene ID | 22919 ncbi.nlm.nih.gov/gene/22919 |
| Ensembl ID | ENSG00000101367 |
| UniProt ID | Q15691 |
| OMIM ID | 603108 |
| HGNC ID | 6890 |
| Aliases | EB1 |
Description
MAPRE1 (Microtubule Associated Protein RP/EB Family Member 1), commonly known as EB1, encodes a member of the RP/EB family of microtubule-associated proteins. This protein localizes to the plus ends of microtubules and regulates microtubule dynamics, cell polarity, and chromosome stability. It interacts with the adenomatous polyposis coli (APC) tumor suppressor and is involved in cell migration and division.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Colorectal Cancer | MAPRE1 overexpression stabilizes microtubule plus ends, promoting cell migration and invasion; interacts with APC in Wnt signaling. | PMID: 12058064, COSMIC |
| Breast Cancer | Elevated MAPRE1 expression correlates with poor prognosis; enhances microtubule dynamics and metastasis. | PMID: 22926525, COSMIC |
| Ciliopathies | MAPRE1 localizes to centrosomes and cilia; mutations disrupt ciliary microtubule organization. | PMID: 20089971, ClinVar |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 28.5 | High |
| Lung | 22.3 | High |
| Colon | 18.7 | Medium |
| Breast | 15.2 | Medium |
| Liver | 10.1 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 35.2 | Cervical cancer cell line; high expression |
| MCF7 | 28.9 | Breast cancer cell line; moderate expression |
| HCT116 | 32.1 | Colorectal cancer cell line; high expression |
| HEK293 | 25.4 | Embryonic kidney; moderate expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1A>G (p.Met1?) | Missense | <0.1% | Loss of start codon; likely loss of function |
| c.487C>T (p.Arg163Trp) | Missense | <0.1% | Alters EB1-APC interaction; reported in ClinVar |
| c.832G>A (p.Glu278Lys) | Missense | <0.1% | Unknown significance; observed in COSMIC |
Mutation functional classification
Loss of Function (LOF)
Rare truncating or start-loss mutations (e.g., p.Met1?) reduce protein expression or disrupt microtubule binding.
Gain of Function (GOF)
Overexpression in cancers (e.g., colorectal, breast) enhances microtubule stability and cell motility; no activating point mutations reported.
Dominant Negative (DN)
Mutations in the EB1-APC interaction domain (e.g., p.Arg163Trp) may disrupt APC-mediated regulation, acting in a dominant-negative manner.
View complete mutation data：
Gene Ontology (GO)
| • microtubule binding (GO:0008017) | • plus-end-directed microtubule binding (GO:0051010) |
| • microtubule cytoskeleton organization (GO:0000226) | • cell division (GO:0051301) |
| • cell migration (GO:0016477) |
Pathways
• Regulation of microtubule dynamics (Reactome: R-HSA-983231)
• APC/C-mediated degradation of cell cycle proteins (Reactome: R-HSA-174178)
• Wnt signaling pathway (KEGG: hsa04310)
Protein Summary
MAPRE1 (EB1) is a 268-amino-acid protein that binds to the plus ends of growing microtubules via its N-terminal calponin homology domain. It recruits various interacting partners (e.g., APC, CLIP-170) to regulate microtubule dynamics, spindle positioning, and cell polarity. The C-terminal EB1 domain mediates dimerization and interactions with other plus-end tracking proteins. Post-translational modifications include phosphorylation and acetylation, which modulate its localization and function.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| MAPRE1 Knockout HEK293 Cell Line | EDJ-KQ7744 | Human | 22919 | Details Get a Quote |
| MAPRE1 Knockout HCT 116 Cell Line | EDJ-KQ33176 | Human | 22919 | Details Get a Quote |
| MAPRE1 Knockout HeLa Cell Line | EDJ-KQ33177 | Human | 22919 | Details Get a Quote |
| MAPRE1 Knockout A-549 Cell Line | EDJ-KQ31853 | Human | 22919 | Details Get a Quote |
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