MAPKAPK5 Gene - MAPK Activated Protein Kinase 5
A serine/threonine kinase involved in stress response, cell proliferation, and tumor suppression.
Gene Information Card
| Symbol | MAPKAPK5 |
|---|---|
| Full Name | MAPK activated protein kinase 5 |
| Gene Type | protein coding |
| Chromosomal Location | 12q24.12 |
| NCBI Gene ID | 8550 ncbi.nlm.nih.gov/gene/8550 |
| Ensembl ID | ENSG00000139099 |
| UniProt ID | Q8IW41 |
| OMIM ID | 606723 |
| HGNC ID | 6889 |
| Aliases | PRAK, MAPKAPK5, DKFZp686K05122 |
Description
MAPKAPK5 (MAPK activated protein kinase 5), also known as PRAK (p38-regulated/activated protein kinase), is a serine/threonine kinase that acts downstream of p38 MAPK signaling. It plays roles in stress-induced responses, cell cycle regulation, senescence, and tumor suppression. MAPKAPK5 phosphorylates substrates such as HSP27 and regulates gene expression. It is widely expressed and implicated in various cancers and inflammatory conditions.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Cancer (various types) | Altered expression/activity; potential tumor suppressor or oncogenic depending on context | COSMIC: mutations found in multiple cancer types; PubMed studies |
| Inflammatory diseases | Regulation of cytokine production via p38 pathway | PubMed studies |
| Cardiovascular disease | Involved in cardiac hypertrophy and stress response | PubMed studies |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 15.2 | Medium |
| Heart | 12.8 | Medium |
| Liver | 8.5 | Low |
| Kidney | 10.3 | Medium |
| Lung | 9.7 | Low |
| Muscle | 7.4 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 18.5 | High expression |
| A549 | 12.3 | Medium |
| MCF7 | 15.0 | High |
| HepG2 | 9.8 | Low |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.112A>G (p.Thr38Ala) | Missense | Rare (<0.1%) | Unknown; may affect kinase activity |
| c.455C>T (p.Pro152Leu) | Missense | Rare | Unknown |
| c.789_790insA (frameshift) | Insertion | Very rare | Likely loss of function |
Mutation functional classification
Loss of Function (LOF)
Frameshift or truncating mutations that abolish kinase activity are likely loss-of-function.
Gain of Function (GOF)
No clear gain-of-function mutations reported; overexpression may mimic activation.
Dominant Negative (DN)
Kinase-dead mutants (e.g., ATP-binding site mutations) can act as dominant-negative in experimental settings.
View complete mutation data：
Gene Ontology (GO)
| • protein serine/threonine kinase activity | • ATP binding |
| • MAP kinase kinase kinase activity | • signal transduction |
| • stress-activated MAPK cascade | • cell cycle |
| • apoptotic process | • regulation of transcription |
Pathways
• p38 MAPK signaling pathway
• MAPK signaling pathway
• Cellular senescence
• TNF signaling pathway
Protein Summary
MAPKAPK5 is a 473-amino acid protein with an N-terminal kinase domain and a C-terminal regulatory region. It is activated by p38 MAPK-mediated phosphorylation at Thr182. It phosphorylates substrates like HSP27 and regulates actin dynamics. It is involved in cell proliferation, differentiation, and stress responses. Subcellular localization is predominantly nuclear and cytoplasmic.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| MAPKAPK5 Knockout HEK293 Cell Line | EDJ-KQ708 | Human | 8550 | Details Get a Quote |
| MAPKAPK5 Knockout A-549 Cell Line | EDJ-KQ19304 | Human | 8550 | Details Get a Quote |
| MAPKAPK5 Knockout HCT 116 Cell Line | EDJ-KQ19305 | Human | 8550 | Details Get a Quote |
| MAPKAPK5 Knockout HeLa Cell Line | EDJ-KQ19306 | Human | 8550 | Details Get a Quote |
