MAPKAPK3
MAPK Activated Protein Kinase 3
Gene Information Card
| Symbol | MAPKAPK3 |
|---|---|
| Full Name | Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 |
| Gene Type | Protein coding |
| Chromosomal Location | 3p21.31 |
| NCBI Gene ID | 7867 ncbi.nlm.nih.gov/gene/7867 |
| Ensembl ID | ENSG00000114738 |
| UniProt ID | Q16644 |
| OMIM ID | 602130 |
| HGNC ID | 6877 |
| Aliases | 3PK, MK3, MAPKAPK-3, MAPKAP3 |
Description
MAPKAPK3 (Mitogen-Activated Protein Kinase-Activated Protein Kinase 3) encodes a serine/threonine protein kinase that is activated by p38 MAPK and to a lesser extent by ERK. It phosphorylates downstream targets involved in stress response, inflammation, cell cycle regulation, and cytoskeletal remodeling. The gene is located on chromosome 3p21.31 and is widely expressed.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Cancer (various) | MAPKAPK3 is implicated in tumorigenesis via p38 MAPK signaling; altered expression affects cell proliferation and apoptosis. | COSMIC, NCBI |
| Inflammatory disorders | MAPKAPK3 regulates cytokine production (e.g., TNF, IL-6) through phosphorylation of transcription factors. | UniProt, NCBI |
| Cardiovascular disease | MAPKAPK3 modulates cardiac hypertrophy and stress responses via p38 pathway. | NCBI, OMIM |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 12.5 | Medium |
| Heart | 15.3 | Medium |
| Liver | 8.2 | Low |
| Lung | 10.1 | Medium |
| Kidney | 11.4 | Medium |
| Testis | 18.7 | High |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 14.2 | Cervical cancer cell line |
| HEK293 | 11.8 | Embryonic kidney cell line |
| A549 | 9.5 | Lung carcinoma cell line |
| MCF7 | 7.3 | Breast cancer cell line |
| K562 | 6.1 | Leukemia cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.101C>T (p.Ala34Val) | Missense | <0.1% | Unknown; likely benign |
| c.457G>A (p.Glu153Lys) | Missense | <0.1% | Unknown; possibly damaging |
| c.788_789insA | Frameshift | <0.1% | Loss of function |
Mutation functional classification
Loss of Function (LOF)
Frameshift mutations (e.g., c.788_789insA) predicted to truncate the kinase domain, reducing catalytic activity.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported in major databases.
Dominant Negative (DN)
Not described for MAPKAPK3.
View complete mutation data：
Gene Ontology (GO)
Pathways
• p38 MAPK signaling pathway (KEGG: hsa04010)
• MAPK signaling pathway (KEGG: hsa04010)
• TNF signaling pathway (KEGG: hsa04668)
• IL-17 signaling pathway (KEGG: hsa04657)
Protein Summary
MAPKAPK3 is a 382-amino acid serine/threonine kinase (UniProt Q16644) with a molecular weight of ~43 kDa. It contains an N-terminal kinase domain and a C-terminal regulatory region. Activation occurs via phosphorylation by p38 MAPK at Thr222 and Ser272. The kinase phosphorylates substrates such as HSP27, CREB, and SRF, influencing stress responses, cytoskeletal dynamics, and gene expression. It is ubiquitously expressed with highest levels in testis and brain.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| MAPKAPK3 Knockout HEK293 Cell Line | EDJ-KQ707 | Human | 7867 | Details Get a Quote |
| MAPKAPK3 Knockout A-549 Cell Line | EDJ-KQ19301 | Human | 7867 | Details Get a Quote |
| MAPKAPK3 Knockout HCT 116 Cell Line | EDJ-KQ19302 | Human | 7867 | Details Get a Quote |
| MAPKAPK3 Knockout HeLa Cell Line | EDJ-KQ19303 | Human | 7867 | Details Get a Quote |
