MAP1LC3B Gene - Microtubule Associated Protein 1 Light Chain 3 Beta
Key autophagy marker and tumor suppressor gene involved in neurodegenerative diseases and cancer
Gene Information Card
| Symbol | MAP1LC3B |
|---|---|
| Full Name | Microtubule Associated Protein 1 Light Chain 3 Beta |
| Gene Type | protein-coding |
| Chromosomal Location | 16q24.2 |
| NCBI Gene ID | 81631 ncbi.nlm.nih.gov/gene/81631 |
| Ensembl ID | ENSG00000140941 |
| UniProt ID | Q9GZQ8 |
| OMIM ID | 609604 |
| HGNC ID | 13352 |
| Aliases | LC3B, MAP1LC3B-a, ATG8F, LC3-II |
Description
MAP1LC3B (Microtubule Associated Protein 1 Light Chain 3 Beta) is a protein-coding gene that encodes a key component of the autophagy machinery. The protein, commonly known as LC3B, is essential for autophagosome formation and maturation. It is widely used as a marker for autophagy due to its conversion from cytosolic LC3-I to lipidated LC3-II during autophagic flux. MAP1LC3B is involved in cellular homeostasis, stress response, and tumor suppression. Dysregulation of MAP1LC3B expression and function is implicated in various cancers, neurodegenerative disorders (e.g., Alzheimer's, Parkinson's), and infectious diseases.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast Cancer | Altered autophagy flux; reduced LC3B expression correlates with poor prognosis | PMID: 23454724 |
| Colorectal Cancer | LC3B overexpression associated with tumor progression and metastasis | PMID: 25944712 |
| Alzheimer's Disease | Impaired autophagy leads to accumulation of LC3B and amyloid-beta | PMID: 21908977 |
| Parkinson's Disease | Defective LC3B-mediated mitophagy contributes to dopaminergic neuron loss | PMID: 22958918 |
| Hepatocellular Carcinoma | LC3B expression levels linked to tumor grade and survival | PMID: 24667604 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 12.5 | Medium |
| Heart | 8.3 | Low |
| Liver | 15.2 | Medium |
| Kidney | 10.1 | Medium |
| Lung | 7.8 | Low |
| Skeletal Muscle | 6.4 | Low |
| Pancreas | 9.9 | Low |
| Spleen | 11.3 | Medium |
| Testis | 18.7 | High |
| Thyroid | 14.0 | Medium |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 22.5 | High expression; used as autophagy model |
| HEK293 | 18.3 | High expression; common for LC3B studies |
| MCF7 | 14.1 | Medium expression; breast cancer line |
| A549 | 12.8 | Medium expression; lung cancer line |
| SH-SY5Y | 16.7 | High expression; neuronal model for autophagy |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1A>G (p.Met1?) | missense | <0.01% | Likely loss of start codon; predicted loss of function |
| c.95C>T (p.Pro32Leu) | missense | <0.01% | Unknown; rare variant in population databases |
| c.244G>A (p.Gly82Arg) | missense | <0.01% | Unknown; rare variant in gnomAD |
| c.331C>T (p.Arg111Cys) | missense | <0.01% | Unknown; rare variant in gnomAD |
Mutation functional classification
Loss of Function (LOF)
Rare missense variants (e.g., p.Met1?) may disrupt protein translation or lipidation, impairing autophagosome formation.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported in MAP1LC3B.
Dominant Negative (DN)
No dominant-negative mutations described for MAP1LC3B.
View complete mutation data：
Gene Ontology (GO)
| • autophagy (GO:0006914) | • macroautophagy (GO:0016236) |
| • autophagosome (GO:0005776) | • pre-autophagosomal structure membrane (GO:0034045) |
| • protein binding (GO:0005515) | • identical protein binding (GO:0042802) |
| • actin binding (GO:0003779) | • microtubule binding (GO:0008017) |
Pathways
• Autophagy - Homo sapiens (human) (KEGG: hsa04140)
• Mitophagy - Homo sapiens (human) (Reactome: R-HSA-5205647)
• Selective autophagy (Reactome: R-HSA-9663891)
• LC3/GABARAP conjugation (Reactome: R-HSA-1632852)
Protein Summary
MAP1LC3B encodes the LC3B protein, a member of the ATG8 family. The protein is synthesized as pro-LC3B, which is cleaved by ATG4 to form cytosolic LC3-I. Upon autophagy induction, LC3-I is conjugated to phosphatidylethanolamine by ATG7, ATG3, and the ATG12-ATG5-ATG16L1 complex to generate lipidated LC3-II, which associates with autophagosomal membranes. LC3-II is a widely used marker for autophagic activity. The protein also interacts with microtubules and actin, linking autophagy to cytoskeletal dynamics. Post-translational modifications include lipidation, acetylation, and ubiquitination, which regulate its function.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| MAP1LC3B2 Knockout HEK293 Cell Line | EDJ-KQ858 | Human | 643246 | Details Get a Quote |
| MAP1LC3B2 Knockout A-549 Cell Line | EDJ-KQ20992 | Human | 643246 | Details Get a Quote |
| MAP1LC3B2 Knockout HCT 116 Cell Line | EDJ-KQ20994 | Human | 643246 | Details Get a Quote |
| MAP1LC3B2 Knockout HeLa Cell Line | EDJ-KQ20995 | Human | 643246 | Details Get a Quote |
| MAP1LC3B Knockout HEK293 Cell Line | EDJ-KQ51769 | Human | 81631 | Details Get a Quote |
| MAP1LC3B Knockout HeLa Cell Line | EDJ-KQ57404 | Human | 81631 | Details Get a Quote |
| MAP1LC3B Knockout A-549 Cell Line | EDJ-KQ65912 | Human | 81631 | Details Get a Quote |
| MAP1LC3B Knockout HCT 116 Cell Line | EDJ-KQ74338 | Human | 81631 | Details Get a Quote |
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