LCMT1: Leucine Carboxyl Methyltransferase 1
A key regulator of protein phosphatase 2A (PP2A) methylation and cellular signaling
Gene Information Card
| Symbol | LCMT1 |
|---|---|
| Full Name | Leucine carboxyl methyltransferase 1 |
| Gene Type | Protein coding |
| Chromosomal Location | 16p12.1 |
| NCBI Gene ID | 51451 ncbi.nlm.nih.gov/gene/51451 |
| Ensembl ID | ENSG00000140564 |
| UniProt ID | O14744 |
| OMIM ID | 609474 |
| HGNC ID | 28987 |
| Aliases | LCMT, CGI-68, PPMT1 |
Description
LCMT1 encodes leucine carboxyl methyltransferase 1, an enzyme that methylates the C-terminal leucine residue of the protein phosphatase 2A (PP2A) catalytic subunit. This methylation is essential for PP2A holoenzyme assembly and activity, thereby regulating diverse cellular processes including cell cycle, apoptosis, and signal transduction.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Cancer (various) | Altered PP2A methylation affects tumor suppressor pathways | PMID: 21900206 |
| Alzheimer's disease | Reduced PP2A methylation linked to tau hyperphosphorylation | PMID: 17984093 |
| Intellectual disability | LCMT1 mutations impair PP2A function in neuronal development | PMID: 28135719 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 12.5 | Medium |
| Heart | 8.3 | Low |
| Liver | 6.1 | Low |
| Kidney | 9.7 | Low |
| Testis | 15.2 | Medium |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 14.0 | High expression |
| HeLa | 11.5 | Moderate expression |
| SH-SY5Y | 13.2 | High expression |
| MCF7 | 9.8 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.442C>T (p.Arg148Trp) | Missense | <0.01% | Reduced methyltransferase activity |
| c.625G>A (p.Gly209Ser) | Missense | <0.01% | Impaired PP2A binding |
| c.1A>G (p.Met1Val) | Start loss | <0.01% | Loss of protein expression |
Mutation functional classification
Loss of Function (LOF)
Missense mutations (e.g., p.Arg148Trp) reduce enzymatic activity and PP2A methylation.
Gain of Function (GOF)
No gain-of-function mutations reported.
Dominant Negative (DN)
No dominant-negative mutations described.
View complete mutation data：
Gene Ontology (GO)
Pathways
• PP2A methylation pathway
• Regulation of protein phosphatase activity
• Wnt signaling pathway
Protein Summary
LCMT1 is a 334-amino acid methyltransferase localized to the cytoplasm and nucleus. It specifically methylates the C-terminal leucine residue of the PP2A catalytic subunit (PPP2CA and PPP2CB), enabling PP2A holoenzyme assembly. This modification is critical for PP2A's role in dephosphorylating key signaling proteins, including those in the Akt, MAPK, and Wnt pathways.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| LCMT1 Knockout HEK293 Cell Line | EDJ-KQ11100 | Human | 51451 | Details Get a Quote |
| LCMT1 Knockout A-549 Cell Line | EDJ-KQ39056 | Human | 51451 | Details Get a Quote |
| LCMT1 Knockout HCT 116 Cell Line | EDJ-KQ39057 | Human | 51451 | Details Get a Quote |
| LCMT1 Knockout HeLa Cell Line | EDJ-KQ37748 | Human | 51451 | Details Get a Quote |
